Effects of Sequence and Base Composition on the CD and TDS Profiles of i-DNA
Open Access
- 25 April 2021
- journal article
- research article
- Published by Wiley in Angewandte Chemie-International Edition
- Vol. 60 (18), 10295-10303
- https://doi.org/10.1002/anie.202016822
Abstract
The i-motif DNA, also known as i-DNA, is a non-canonical DNA secondary structure formed by cytosine-rich sequences, consisting of two intercalated parallel-stranded duplexes held together by hemi-protonated cytosine-cytosine(+) (C:C+) base pairs. The growing interest in the i-DNA structure as a target in anticancer therapy increases the need for tools for a rapid and meaningful interpretation of the spectroscopic data of i-DNA samples. Herein, we analyzed the circular dichroism (CD) and thermal difference UV-absorbance spectra (TDS) of 255 DNA sequences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i-DNA-forming sequences.Funding Information
- Associazione Italiana per la Ricerca sul Cancro (IG 18695)
- Regione Campania (B61G18000470007)
- National Natural Science Foundation of China (21977045)
- Nanjing University (020514912216)
- Fundamental Research Funds for the Central Universities (02051430210)
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