Characterization of Novel Bacteriophage AhyVDH1 and Its Lytic Activity Against Aeromonas hydrophila
- 11 November 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Current Microbiology
- Vol. 78 (1), 329-337
- https://doi.org/10.1007/s00284-020-02279-7
Abstract
Phage therapy is an alternative approach to overcome the problem of multidrug-resistant bacteria. Here, a novel bacteriophage AhyVDH1, which infects Aeromonas hydrophila 4572, was isolated and its morphology, one-step growth curve, lytic activity, stability under various conditions, and genome were investigated. Transmission electron microscopy revealed that AhyVDH1 has an icosahedral head 49 nm in diameter and a contractile tail 127 nm in length, suggesting that it belongs to the family Myoviridae. AhyVDH1 showed strong adsorption to the surface of A. hydrophila 4572 (90% in 10 min). The latent period of AhyVDH1 was shown to be 50 min, and the burst size was 274 plaque-forming unit/infected cell. AhyVDH1 was stable at 30 °C for 1 h and lost infectivity after20 min of heating at 60 °C. Infectivity remained unaffected at pH 6–7 for 1 h, while the bacteriophage was inactivated at pH < 4 or > 11. AhyVDH1 has a 39,175-bp genome, with a 58% G + C content and 59 open reading frames. BLAST analysis indicated that the genome sequence of phage AhyVDH1 was related to that of Aeromonas phage Ahp2. Both time and MOI-dependent in vitro A. hydrophila growth inhibition were observed with AhyVDH1.Re-growth of the host bacteria appeared about 12 h after treatment, suggesting its potential therapeutic value in treating A. hydrophila infections, but phage cocktails should be developed.Keywords
Funding Information
- National Natural Science Foundation of China (31660042, 31660001, 32071570)
- National Natural Science Foundation of China (31660089)
- Yunnan Provincial Science and Technology Department (2018IA100)
This publication has 38 references indexed in Scilit:
- SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell SequencingJournal of Computational Biology, 2012
- Easyfig: a genome comparison visualizerBioinformatics, 2011
- Copper inducing effect on laccase production of white rot fungi native from Misiones (Argentina)Enzyme and Microbial Technology, 2010
- Phage therapyDrug Discovery Today, 2009
- Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophilaFish & Shellfish Immunology, 2008
- Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilumApplied and Environmental Microbiology, 2008
- Aeromonas hydrophila and Aeromonas sobria bacteremia: Rare pathogens of infection in a burn patientClinical Dermatology and Surgery, 2007
- Isolation and characterization of a Lactobacillus plantarum bacteriophage, ΦJL-1, from a cucumber fermentationInternational Journal of Food Microbiology, 2003
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- The isolation, identification, and systematics of the motile Aeromonas speciesAnnual Review of Fish Diseases, 1994