Cinnamomi ramulus inhibits cancer cells growth by inducing G2/M arrest
Open Access
- 17 March 2023
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Pharmacology
- Vol. 14, 1121799
- https://doi.org/10.3389/fphar.2023.1121799
Abstract
Cinnamomum ramulus (CR) is one of the most widely used traditional Chinese medicine (TCM) with anti-cancer effects. Analyzing transcriptomic responses of different human cell lines to TCM treatment is a promising approach to understand the unbiased mechanism of TCM. This study treated ten cancer cell lines with different CR concentrations, followed by mRNA sequencing. Differential expression (DE) analysis and gene set enrichment analysis (GSEA) were utilized to analyze transcriptomic data. Both methods suggested the Cell cycle pathway was the most perturbated pathway by CR across these cell lines. By analyzing the clinical significance and prognosis of G2/M related genes (PLK1, CDK1, CCNB1, and CCNB2) in various cancer tissues, we found that they were up-regulated in most cancer types, and their down-regulation showed better overall survival rates in cancer patients. Finally, in vitro experiments validation on A549, Hep G2, and HeLa cells suggested that CR can inhibit cell growth by suppressing the PLK1/CDK1/ Cyclin B axis. This is the first study to apply transcriptomic analysis to investigate the cancer cell growth inhibition of CR on various human cancer cell lines. The core effect of CR on ten cancer cell lines is to induce G2/M arrest by inhibiting the PLK1/CDK1/Cyclin B axis.This publication has 52 references indexed in Scilit:
- Foeniculum vulgare: A comprehensive review of its traditional use, phytochemistry, pharmacology, and safetyArabian Journal of Chemistry, 2016
- The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivityNature, 2012
- The cell cycle and cancerThe Journal of Pathology, 2011
- Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathwayJournal of Experimental & Clinical Cancer Research, 2011
- The Role of Traditional Chinese Herbal Medicines in Cancer Therapy – from TCM Theory to Mechanistic InsightsPlanta Medica, 2010
- Cyclin-dependent kinases and cell-cycle transitions: does one fit all?Nature Reviews Molecular Cell Biology, 2008
- The Human Protein Atlas—a tool for pathologyThe Journal of Pathology, 2008
- The Connectivity Map: Using Gene-Expression Signatures to Connect Small Molecules, Genes, and DiseaseScience, 2006
- Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profilesProceedings of the National Academy of Sciences of the United States of America, 2005
- Cell cycle checkpoint signaling through the ATM and ATR kinasesGenes & Development, 2001