Stacked graphene with nanoscale wrinkles supports osteogenic differentiation of human adipose-derived stromal cells
- 28 January 2021
- journal article
- research article
- Published by IOP Publishing in 2D Materials
- Vol. 8 (2), 025034
- https://doi.org/10.1088/2053-1583/abe105
Abstract
Based on the concept that microenvironment and physical stimuli regulate the cell behaviors like proliferation, migration, and differentiation, this study was conducted to investigate whether nanoscale spacing by stacked graphene film affect osteogenic differentiation of human adipose-derived mesenchymal stromal cells (hADSCs). The graphene films were synthesized by a chemical vapor deposition method, followed by etching and rinsing process to fabricate single or 3-, 5-, and 7-multilayers. The height and width of wrinkles of the graphene were confirmed by SEM and atomic force microscopy (AFM), ranging from 1.5 to 12.5 nm and from 30 to 100 nm, respectively. Osteogenic differentiation was significantly (p < 0.0001) promoted as the stacking layer increased. Immunofluorescent imaging and osteogenesis-related gene expression showed which increment was saturated from three layers. The calcium deposits and expression of osteogenesis-related genes (Runt-related transcription factor 2 and Osteocalcin) were highest in the three layers. In the hADSCs cultured on the three layers, the intensity of protein expression levels of filamentous actin (F-actin) was significantly increased (p = 0.0319) and focal adhesion kinase/extracellular signal-regulated kinase signal related genes were concomitantly activated. These results demonstrated that multilayer-stacked graphene creating nanoscale spaces promotes calcium deposit and cytoskeletal integrity in hADSC-related, in vitro-osteogenesis.Keywords
This publication has 39 references indexed in Scilit:
- Quasi-3D morphology and modulation of focal adhesions of human adult stem cells through combinatorial concave elastomeric surfaces with varied stiffnessSoft Matter, 2019
- Substrate stiffness- and topography-dependent differentiation of annulus fibrosus-derived stem cells is regulated by Yes-associated proteinActa Biomaterialia, 2019
- Modulation of Mesenchymal Stem Cells Mechanosensing at Fluid Interfaces by Tailored Self‐Assembled Protein MonolayersSmall, 2019
- Distinct Mechanosensing of Human Neural Stem Cells on Extremely Limited Anisotropic Cellular ContactACS Applied Materials & Interfaces, 2018
- Graphene quantum dots prevent α-synucleinopathy in Parkinson’s diseaseNature Nanotechnology, 2018
- Harnessing nanotopography and integrin–matrix interactions to influence stem cell fateNature Materials, 2014
- Materials as stem cell regulatorsNature Materials, 2014
- Graphene and graphene oxide as new nanocarriers for drug delivery applicationsActa Biomaterialia, 2013
- The control of human mesenchymal cell differentiation using nanoscale symmetry and disorderNature Materials, 2007
- The guidance of human mesenchymal stem cell differentiation in vitro by controlled modifications to the cell substrateBiomaterials, 2006