Antibacterial Activity of Prunus Scoparia Root Methanol Extract against Most Common Burn Wound Pathogens
Open Access
- 1 November 2020
- journal article
- research article
- Vol. 45 (6), 444-450
- https://doi.org/10.30476/ijms.2019.82071
Abstract
Background: Burn wound infection and sepsis are serious medical conditions requiring prompt intervention. Plants are a good natural source for the development of novel, safe, and costeffective antibacterial agents. The objective of the present study was to assess the antibacterial potential of aqueous, chloroform, and methanol extracts of the Prunus scoparia (P. scoparia) root against the most common burn wound pathogens. Methods: The present experimental study was conducted at Shiraz University of Medical Sciences (Shiraz, Iran) during 2018-2019. The antibacterial activity of the total plant extract was assayed using the broth microdilution method. Fractionation was performed using a separation funnel and solvents with different polarities. Broth microdilution and agar well diffusion assays were performed to determine the antibacterial potential of the obtained fractions. Quantitative and qualitative phytochemical analyses were performed to confirm the presence of secondary metabolites in both the total extract and the fractions. Results: Methanolic extract of P. scoparia root exhibited antibacterial activity against all tested bacterial strains, especially against Methicillin-resistant Staphylococcus aureus (MRSA) isolates. This extract, compared to the aqueous and chloroformic extracts, exhibited the presence of active antibacterial compounds. The quantitative and qualitative results of phytochemical screening showed that phenols and flavonoids were the main antibacterial compounds in the methanolic extract of the plant. Conclusion: For the first time, we demonstrated the antibacterial activity of the P. scoparia root against MRSA isolates and other common burn wound pathogens.Keywords
This publication has 19 references indexed in Scilit:
- Sepsis in the burn patient: a different problem than sepsis in the general populationBurns & Trauma, 2017
- Recent trends in burn epidemiology worldwide: A systematic reviewBurns, 2016
- Effect of Roasting on Physicochemical Properties of Wild Almonds (Amygdalus scoparia)Journal of Oil & Fat Industries, 2016
- Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. varietiesAsian Pacific Journal of Tropical Biomedicine, 2016
- TOTAL PHENOLIC CONTENT, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF THE EXTRACT OF EPHEDRA PROCERA FISCH. ET MEY.2015
- Gut microbiota and environment in patients with major burns – A preliminary reportBurns, 2014
- Comparison of phenolic acids and flavonoids in black garlic at different thermal processing stepsJournal of Functional Foods, 2013
- Garlic ointment inhibits biofilm formation by bacterial pathogens from burn woundsJournal of Medical Microbiology, 2012
- Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acidsPharmaceutical Biology, 2011
- Epidemiology of burn injuries in the East Mediterranean Region: a systematic reviewBMC Public Health, 2010