IgE antibody repertoire in nasal secretions of children and adults with seasonal allergic rhinitis: A molecular analysis
Open Access
- 1 April 2020
- journal article
- research article
- Published by Wiley in Pediatric Allergy and Immunology
- Vol. 31 (3), 273-280
- https://doi.org/10.1111/pai.13148
Abstract
Background There is growing interest both in testing IgE in nasal secretions (NS) and in molecular diagnosis of seasonal allergic rhinitis (SAR). Yet, the reliability of nasal IgE detection with the newest molecular assays has never been assessed in a large cohort of pollen allergic patients. Objective To investigate with microarray technology and compare the repertoires of specific IgE (sIgE) antibodies in NS and sera of a large population of children and adults with SAR. Methods Nasal secretions were collected with an absorbent device (Merocel 2000 (R), Medtronic) and a minimal dilution procedure from 90 children and 71 adults with SAR. Total IgE (tIgE) (ImmunoCAP, Thermo Fisher Scientific (TFS)) and sIgE antibodies against 112 allergen molecules (ISAC-112, TFS) were measured in NS and serum. Results Nasal sIgE was detectable in 68.3% of the patients. The detected nasal sIgE antibodies recognized airborne (88%), vegetable (10%), and animal food or other (<1%) allergen molecules. The prevalence and average levels of sIgE in NS and serum were highly interrelated at population level. A positive nasal sIgE antibody to a given molecule predicted the detection of the same antibody in the patient's serum with a specificity of 99.7% and a sensitivity of 40%. Conclusions The concentration of sIgE is much lower in nasal secretions than in the serum. sIgE assays with very high analytical sensitivity and sampling methods with minimal dilution will be therefore needed to validate nasal secretions as alternative to serum in testing the sIgE repertoire.This publication has 27 references indexed in Scilit:
- Advances in allergen-microarray technology for diagnosis and monitoring of allergy: The MeDALL allergen-chipMethods, 2013
- Detecting local immunoglobulin E from mucosal brush biopsy of the inferior turbinates using microarray analysisInternational Forum of Allergy & Rhinology, 2012
- The who, where, and when of IgE in allergic airway diseaseJournal of Allergy and Clinical Immunology, 2012
- A role for measurement of nasal IgE antibodies in diagnosis of Alternaria-induced rhinitis in childrenAllergologia et Immunopathologia, 2011
- Seasonal idiopathic rhinitis with local inflammatory response and specific IgE in absence of systemic responseAllergy, 2008
- Local IgE production and positive nasal provocation test in patients with persistent nonallergic rhinitisJournal of Allergy and Clinical Immunology, 2007
- Allergen Drives Class Switching to IgE in the Nasal Mucosa in Allergic RhinitisThe Journal of Immunology, 2005
- Collection of nasal secretions for immunological analysisEuropean Archives of Oto-Rhino-Laryngology, 2003
- LOCAL PRODUCTION OF SPECIFIC IgE ANTIBODIES IN ALLERGIC-RHINITIS PATIENTS WITH NEGATIVE SKIN TESTSThe Lancet, 1975
- IgE antibodies in nasal secretions of ragweed-allergic subjectsJournal of Allergy, 1970