Preemptive Stem Cells Ameliorate Neuropathic Pain in Rats: A Central Component of Preemptive Analgesia
- 16 February 2021
- journal article
- research article
- Published by Oxford University Press (OUP) in Microscopy and Microanalysis
- Vol. 27 (2), 450-456
- https://doi.org/10.1017/s1431927621000076
Abstract
The present study aims to investigate the efficacy of intravenously injected mesenchymal stem cells (MSCs) in treating neuropathic pain either before or after its induction by a chronic constriction injury (CCI) model. Rats were divided into four groups: control group, neuropathic group, and treated groups (pre and postinduction) with i.v. mononuclear cells (106 cell/mL). For these rats, experimental testing for both thermal and mechanical hyperalgesia was evaluated. The cerebral cortex of the rats was dissected, and immunohistochemical analysis using anti-proliferating cell nuclear antigen (PCNA), CD117, nestin, and glial fibrillary acidic protein was performed. Our results showed that a single injection of MSCs (either preemptive/or post-CCI) produced equipotent effects on allodynia, mechanical hyperalgesia, and thermal response. Immunohistochemical analysis showed that the stem cells have reached the cerebral cortex. The injected group with MSCs before CCI showing few stem cells expressed PCNA, CD117, and nestin in the cerebral cortex. The group injected with MSCs after CCI, showing numerous recently proliferated CD117-, nestin-, PCNA-positive stem cells in the cerebral cortex. In conclusion, our findings demonstrate that the most probable effect of i.v. stem cells is the central anti-inflammatory effect, which opens concerns about how stem cells circulating in systemic administration to reach the site of injury.This publication has 30 references indexed in Scilit:
- Low Intensity Focused Ultrasound Modulation of Vincristine Induced NeuropathyNeuroscience, 2020
- Peripheral antinociception induced by ketamine is mediated by the endogenous opioid systemEuropean Journal of Pharmacology, 2019
- Stem cell therapy in pain medicineThe Korean Journal of Pain, 2019
- Intrathecal Administration of Mesenchymal Stem Cells Reduces the Reactive Oxygen Species and Pain Behavior in Neuropathic RatsThe Korean Journal of Pain, 2014
- A preliminary report on stem cell therapy for neuropathic pain in humansJournal of Pain Research, 2014
- The Effects of Dexmedetomidine Alone and in Combination with Tramadol or Amitriptyline in a Neuropathic Pain ModelPain Physician, 2014
- Potential role of stem cells for neuropathic pain disordersNeurosurgical Focus, 2013
- Neuroinflammation and the generation of neuropathic painBritish Journal of Anaesthesia, 2013
- Gene therapy: can neural stem cells deliver?Nature Reviews Neuroscience, 2006
- Radial glia diversity: A matter of cell fateGlia, 2003