Intraspecific nucleotide divergence in Saccharomycodes ludwigii, and proposal of Saccharomycodes pseudoludwigii sp. nov, a new apiculate yeast isolated from China
- 23 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Antonie van Leeuwenhoek
- Vol. 114 (5), 553-559
- https://doi.org/10.1007/s10482-021-01540-8
Abstract
The six synonyms currently accepted under Saccharomycodes ludwigii were investigated for by phenotypic properties, however, the sequence diversity of the rRNA and protein coding genes have not yet been determined. Nine strains including the type strains of synonyms of S. ludwigii deposited in the CBS yeast collection, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands, were analyzed using a multi-locus sequence analysis (MLSA) approach that included sequences of 18S ribosomal RNA (rRNA), the D1/D2 domains of the 26S rRNA, the ITS region (including the 5.8S rRNA) and fragments of genes encoding the largest subunit of the RNA polymerase II (RPB1 and RPB2) and translation elongation factor 1-α (TEF1). Our results showed that the nine strains have identical D1/D2, 18S and RPB2 sequences and similar ITS, RPB1 and TEF1 sequences, which indicated that they are conspecific. In addition, a novel species of Saccharomycodes, S. pseudoludwigii sp. nov. (type CGMCC 2.4526 T) that was isolated from fruit and tree bark in China, is proposed. The MycoBank number of this new species is MB 811,650.Keywords
Funding Information
- National Natural Science Foundation of China (No.31770018, No. 31961133020)
- Talents Research Project of Hebei University (No. 521000981388)
This publication has 13 references indexed in Scilit:
- International Code of Nomenclature for algae, fungi, and plantsRegnum Vegetabile, 2018
- MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger DatasetsMolecular Biology and Evolution, 2016
- Moniliellomycetes and Malasseziomycetes, two new classes in UstilaginomycotinaPersoonia - Molecular Phylogeny and Evolution of Fungi, 2014
- Surprisingly diverged populations of Saccharomyces cerevisiae in natural environments remote from human activityMolecular Ecology, 2012
- Methods for Isolation, Phenotypic Characterization and Maintenance of YeastsPublished by Elsevier BV ,2011
- Saccharomycodes E.C.Published by Elsevier BV ,2011
- Molecular phylogeny of basidiomycetous yeasts in theCryptococcus luteoluslineage (Tremellales) based on nuclear rRNA and mitochondrial cytochromebgene sequence analyses: proposal ofDerxomycesgen. nov. andHannaellagen. nov., and description of eight novelDerxomycesspeciesFEMS Yeast Research, 2008
- Application of Phylogenetic Networks in Evolutionary StudiesMolecular Biology and Evolution, 2005
- Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequencesAntonie van Leeuwenhoek, 1998
- Saccharomycodes E.C. HansenPublished by Elsevier BV ,1998