Searching for Novel Candidate Biomarkers of RLS in Blood by Proteomic Analysis
Open Access
- 1 June 2021
- journal article
- research article
- Published by Taylor & Francis Ltd in Nature and Science of Sleep
- Vol. ume 13, 873-883
- https://doi.org/10.2147/nss.s311801
Abstract
Purpose: We performed comparative proteomic analyses of blood of patients with RLS and healthy individuals aiming to identify potential biomarker and therapeutic target candidate for RLS. Patients and Methods: Blood serum samples from 12 patients with a clinical diagnosis of RLS (8 females and 4 males, with a mean age of 68.52 years) and 10 healthy controls (5 females and 5 males, with a mean age of 67.61 years) underwent proteomic profiling by liquid chromatography coupled with tandem mass spectrometry. Pathway analysis incorporating protein–protein interaction networks was carried out to identify pathological processes linked to the differentially expressed proteins. Results: We quantified 272 proteins in patients with RLS and healthy controls, of which 243 were shared. Five proteins – apolipoprotein C-II, leucine-rich alpha-2-glycoprotein 1, FLJ92374, extracellular matrix protein 1, and FLJ93143 – were substantially increased in RLS patients, whereas nine proteins – vitamin D-binding protein, FLJ78071, alpha-1-antitrypsin, CD5 antigen-like, haptoglobin, fibrinogen alpha chain, complement factor H-related protein 1, platelet factor 4, and plasma protease C1 inhibitor – were decreased. Bioinformatics analyses revealed that these proteins were linked to 1) inflammatory and immune response, and complement activation, 2) brain-related development, cell aging, and memory disorders, 3) pregnancy and associated complications, 4) myocardial infarction, and 5) reactive oxygen species generation and subsequent diabetes mellitus. Conclusion: Our findings shed light on the multifactorial nature of RLS and identified a set of circulating proteins that may have clinical importance as biomarkers and therapeutic targets.Keywords
This publication has 85 references indexed in Scilit:
- Leucine-Rich α2-Glycoprotein Is a Novel Biomarker of Neurodegenerative Disease in Human Cerebrospinal Fluid and Causes Neurodegeneration in Mouse Cerebral CortexPLOS ONE, 2013
- Proteomic analysis of the cerebrospinal fluid of patients with restless legs syndrome/Willis-Ekbom diseaseFluids and Barriers of the CNS, 2013
- Platelets as initiators and mediators of inflammation at the vessel wallThrombosis Research, 2011
- Complement and viral pathogenesisVirology, 2011
- Blood-based diagnostics of traumatic brain injuriesExpert Review of Molecular Diagnostics, 2011
- Cardiovascular and musculskeletal co-morbidities in patients with alpha 1 antitrypsin deficiencyRespiratory Research, 2010
- Complement: a key system for immune surveillance and homeostasisNature Immunology, 2010
- Biological activities of C1 inhibitorMolecular Immunology, 2008
- CSF Multianalyte Profile Distinguishes Alzheimer and Parkinson DiseasesAmerican Journal of Clinical Pathology, 2008
- Acute-Phase Proteins and Other Systemic Responses to InflammationThe New England Journal of Medicine, 1999