Postprandial Endotoxin Transporters LBP and sCD14 Differ in Obese vs. Overweight and Normal Weight Men during Fat-Rich Meal Digestion
Open Access
- 18 June 2020
- Vol. 12 (6), 1820
- https://doi.org/10.3390/nu12061820
Abstract
Circulating levels of lipopolysaccharide-binding protein (LBP) and soluble cluster of differentiation 14 (sCD14) are recognized as clinical markers of endotoxemia. In obese men, postprandial endotoxemia is modulated by the amount of fat ingested, being higher compared to normal-weight (NW) subjects. Relative variations of LBP/sCD14 ratio in response to overfeeding are also considered important in the inflammation set-up, as measured through IL-6 concentration. We tested the hypothesis that postprandial LBP and sCD14 circulating concentrations differed in obese vs. overweight and NW men after a fat-rich meal. We thus analyzed the postprandial kinetics of LBP and sCD14 in the context of two clinical trials involving postprandial tests in normal-, over-weight and obese men. In the first clinical trial eight NW and 8 obese men ingested breakfasts containing 10 vs. 40 g of fat. In the second clinical trial, 18 healthy men were overfed during 8 weeks. sCD14, LBP and Il-6 were measured in all subjects during 5 h after test meal. Obese men presented a higher fasting and postprandial LBP concentration in plasma than NW men regardless of fat load, while postprandial sCD14 was similar in both groups. Irrespective of the overfeeding treatment, we observed postprandial increase of sCD14 and decrease of LBP before and after OF. In obese individuals receiving a 10 g fat load, whereas IL-6 increased 5h after meal, LBP and sCD14 did not increase. No direct association between the postprandial kinetics of endotoxemia markers sCD14 and LBP and of inflammation in obese men was observed in this study.Keywords
This publication has 41 references indexed in Scilit:
- High‐fat meal induced postprandial inflammationMolecular Nutrition & Food Research, 2013
- Determinants of Serum Concentrations of Lipopolysaccharide-Binding Protein (LBP) in the Adult Population: The Role of ObesityPLOS ONE, 2013
- Endotoxin increase after fat overload is related to postprandial hypertriglyceridemia in morbidly obese patientsJournal of Lipid Research, 2012
- Emulsified lipids increase endotoxemia: possible role in early postprandial low-grade inflammationThe Journal of Nutritional Biochemistry, 2011
- A Marker of Endotoxemia Is Associated With Obesity and Related Metabolic Disorders in Apparently Healthy ChineseDiabetes Care, 2010
- Chylomicrons promote intestinal absorption of lipopolysaccharidesJournal of Lipid Research, 2009
- A high-fat meal induces low-grade endotoxemia: evidence of a novel mechanism of postprandial inflammationThe American Journal of Clinical Nutrition, 2007
- Inflammation and metabolic disordersNature, 2006
- Potential Role of Endotoxin as a Proinflammatory Mediator of AtherosclerosisArteriosclerosis, Thrombosis, and Vascular Biology, 2004
- Lipopolysaccharide (LPS)-binding protein accelerates the binding of LPS to CD14.The Journal of Experimental Medicine, 1994