Second-Generation Dendrimers with Chondroitin Sulfate Type-E Disaccharides as Multivalent Ligands for Langerin
- 1 July 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 21 (7), 2726-2734
- https://doi.org/10.1021/acs.biomac.0c00476
Abstract
Chondroitin sulfate type-E (CS-E) is a sulfated polysaccharide that shows several interesting biological activities, such as modulation of the neuronal growth factor signaling and its interaction with langerin, a C-type lectin with a crucial role in the immunological system. However, applications of CS-E are hampered by the typical heterogeneous structure of the natural polysaccharide. Well-defined, homogeneous CS-E analogues are highly demanded. Here, we report the synthesis of monodispersed, structurally well-defined second-generation glycodendrimers displaying up to 18 CS-E disaccharide units. These complex multivalent systems have a molecular weight and a number of disaccharide repeating units comparable with those of the natural polysaccharides. In addition, surface plasmon resonance experiments revealed a calcium-independent interaction between these glycodendrimers and langerin, in the micromolar range, highlighting the utility of these compounds as CS-E mimetics.Funding Information
- Agence Nationale de la Recherche (ANR-15-IDEX-02)
- Ministerio de Ciencia, Innovaci?n y Universidades (CTQ2017-86265-P, PGC2018-099497-B-100)
- Junta de Andaluc?a (FQM-1303)
- European Regional Development Fund (PGC2018-099497-B-100)
- Instituto de Salud Carlos III (RD16/0006/0011)
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