Regulation of Cell Volume by Nanosecond Pulsed Electric Fields
- 14 September 2021
- journal article
- research article
- Published by American Chemical Society (ACS) in The Journal of Physical Chemistry B
- Vol. 125 (38), 10692-10700
- https://doi.org/10.1021/acs.jpcb.1c06058
Abstract
Stimulation of cells by nanosecond pulsed electric fields (nsPEFs) has attracted attention as a technology for medical applications such as cancer treatment. nsPEFs have been shown to affect intracellular environments without significant damage to cell membranes; however, the mechanism underlying the effect of nsPEFs on cells remains unclear. In this study, we constructed electrodes for applying nsPEFs and analyzed the change in volume of a single cell due to nsPEFs using fluorescence and Raman microscopy. It was shown that the direction of the change depended on the applied electric field; expansion due to the influx of water was observed at high electric field, and cell shrinkage was observed at low electric field. The change in cell volume was correlated to the change in the intracellular Ca2+ concentration, and nsPEFs-induced shrinking was not observed when the Ca2+-free medium was used. This result suggests that the cell shrinkage is related to the regulatory volume decrease where the cell adjusts the increase in intracellular Ca2+ concentration, inducing the efflux of ions and water from the cell.Keywords
Funding Information
- Ministry of Education, Culture, Sports, Science and Technology (JP17H05869, JP19H02666, JP20H04689)
This publication has 45 references indexed in Scilit:
- Recruitment of the intracellular Ca2+ by ultrashort electric stimuli: The impact of pulse durationCell Calcium, 2013
- Nanosecond Pulsed Electric Field Inhibits Cancer Growth Followed by Alteration in Expressions of NF-κB and Wnt/β-Catenin Signaling MoleculesPLOS ONE, 2013
- Two Modes of Cell Death Caused by Exposure to Nanosecond Pulsed Electric FieldPLOS ONE, 2013
- Thresholds for Phosphatidylserine Externalization in Chinese Hamster Ovarian Cells following Exposure to Nanosecond Pulsed Electrical Fields (nsPEF)PLOS ONE, 2013
- Induction of Cell Death Mechanisms and Apoptosis by Nanosecond Pulsed Electric Fields (nsPEFs)Cells, 2013
- Label-free Raman observation of cytochrome c dynamics during apoptosisProceedings of the National Academy of Sciences of the United States of America, 2011
- Nanosecond pulsed electric field induced cytoskeleton, nuclear membrane and telomere damage adversely impact cell survivalBioelectrochemistry, 2011
- Cold plasma selectivity and the possibility of a paradigm shift in cancer therapyBritish Journal of Cancer, 2011
- Adipose-Derived Stem Cells in Functional Bone Tissue Engineering: Lessons from Bone MechanobiologyTissue Engineering, Part B: Reviews, 2011
- Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulsesBiochimica et Biophysica Acta (BBA) - Biomembranes, 2011