A Novel and Divergent Gyrovirus with Unusual Genomic Features Detected in Wild Passerine Birds from a Remote Rainforest in French Guiana
Open Access
- 11 December 2019
- Vol. 11 (12), 1148
- https://doi.org/10.3390/v11121148
Abstract
Sequence-independent amplification techniques have become important tools for virus discovery, metagenomics, and exploration of viral diversity at the global scale, especially in remote areas. Here, we describe the detection and genetic characterization of a novel gyrovirus, named GyV11, present in cloacal, oral, and blood samples from neotropical wild birds in French Guiana. The molecular epidemiology revealed the presence of GyV11 only in passerine birds from three different species at a low prevalence (0.73%). This is the first characterization and prevalence study of a gyrovirus carried out in resident wild bird populations in a remote region, and provides evidence of the fecal–oral route transmission and local circulation of the virus. The molecular phylogeny of gyroviruses reveals the existence of two distinct gyrovirus lineages in which GyV11 is phylogenetically distinct from previously reported gyroviruses. Furthermore, GyV11 is placed basal in the gyrovirus phylogeny, likely owing to its ancestral origin and marked divergence. This study also provides important insights into the ecology, epidemiology, and genomic features of gyroviruses in a remote neotropical rainforest. The pathogenesis of this virus in avian species or whether GyV11 can infect humans and/or chickens needs to be further investigated.Keywords
Funding Information
- Agence Nationale de la Recherche (CEBA:ANR-10-LABX-25-01)
- UCM (CT27/16-CT28/16)
This publication has 50 references indexed in Scilit:
- Human Gyrovirus in Healthy Blood Donors, FranceEmerging Infectious Diseases, 2013
- Epidemiology of chicken anemia virus in Central African Republic and CameroonVirology Journal, 2012
- Human Gyrovirus DNA in Human Blood, ItalyEmerging Infectious Diseases, 2012
- Bayesian Phylogenetics with BEAUti and the BEAST 1.7Molecular Biology and Evolution, 2012
- Analysis of chicken anemia virus genome: evidence of intersubtype recombinationVirology Journal, 2011
- The Diversity of Torque Teno Viruses: In Vitro Replication Leads to the Formation of Additional Replication-Competent Subviral MoleculesJournal of Virology, 2011
- Full-length transcriptome assembly from RNA-Seq data without a reference genomeNature Biotechnology, 2011
- New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0Systematic Biology, 2010
- Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifsProceedings of the National Academy of Sciences of the United States of America, 2009
- DNAPlotter: circular and linear interactive genome visualizationBioinformatics, 2008