Development of a potent Zika virus vaccine using self-amplifying messenger RNA
Open Access
- 7 August 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 6 (32), eaba5068
- https://doi.org/10.1126/sciadv.aba5068
Abstract
Zika virus (ZIKV) is the cause of a pandemic associated with microcephaly in newborns and Guillain-Barre syndrome in adults. Currently, there are no available treatments or vaccines for ZIKV, and the development of a safe and effective vaccine is a high priority for many global health organizations. We describe the development of ZIKV vaccine candidates using the self-amplifying messenger RNA (SAM) platform technology delivered by cationic nanoemulsion (CNE) that allows bedside mixing and is particularly useful for rapid responses to pandemic outbreaks. Two immunizations of either of the two lead SAM (CNE) vaccine candidates elicited potent neutralizing antibody responses to ZIKV in mice and nonhuman primates. Both SAM (CNE) vaccines protected these animals from ZIKV challenge, with one candidate providing complete protection against ZIKV infection in nonhuman primates. The data provide a preclinical proof of concept that a SAM (CNE) vaccine candidate can rapidly elicit protective immunity against ZIKV.Funding Information
- Intramural funding from the NIAID
This publication has 47 references indexed in Scilit:
- RNA: The new revolution in nucleic acid vaccinesSeminars in Immunology, 2013
- Nonviral delivery of self-amplifying RNA vaccinesProceedings of the National Academy of Sciences of the United States of America, 2012
- A West Nile Virus DNA Vaccine Utilizing a Modified Promoter Induces Neutralizing Antibody in Younger and Older Healthy Adults in a Phase I Clinical TrialThe Journal of Infectious Diseases, 2011
- The nuclear localization signal sequence of porcine circovirus type 2 ORF2 enhances intracellular delivery of plasmid DNAArchiv für die gesamte Virusforschung, 2011
- Zika Virus Outbreak on Yap Island, Federated States of MicronesiaThe New England Journal of Medicine, 2009
- Structural insights into the mechanisms of antibody-mediated neutralization of flavivirus infection: Implications for vaccine developmentCell Host & Microbe, 2008
- A rapid and quantitative assay for measuring antibody-mediated neutralization of West Nile virus infectionVirology, 2006
- West Nile Virus Recombinant DNA Vaccine Protects Mouse and Horse from Virus Challenge and Expresses In Vitro a Noninfectious Recombinant Antigen That Can Be Used in Enzyme-Linked Immunosorbent AssaysJournal of Virology, 2001
- Molecular Organization of a Recombinant Subviral Particle from Tick-Borne Encephalitis VirusMolecular Cell, 2001
- Recombinant vaccinia virus producing the prM and E proteins of yellow fever virus protects mice from lethal yellow fever encephalitisVirology, 1992