Effects of high-intensity intermittent exercise on the contractile properties of human type I and type II skeletal muscle fibers
- 26 March 2020
- journal article
- research article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 128 (5), 1207-1216
- https://doi.org/10.1152/japplphysiol.00014.2020
Abstract
In vitro studies have shown that alterations in redox state can cause a range of opposing effects on the properties of the contractile apparatus in skeletal muscle fibers. To test whether and how redox changes occurring in vivo affect the contractile properties, vastus lateralis muscle fibers from seven healthy young adults were examined at rest (PRE) and following (POST) high-intensity intermittent cycling exercise. Individual mechanically-skinned muscle fibers were exposed to heavily buffered solutions at progressively higher free [Ca2+] to determine their force-Ca2+ relationship. Following acute exercise, Ca2+ sensitivity was significantly decreased in type I fibers (by 0.06 pCa unit) but not in type II fibers (0.01 pCa unit). Specific force decreased after the exercise in type II fibers (-18%), but was unchanged in type I fibers. Treatment with the reducing agent dithiothreitol (DTT) caused a small decrease in Ca2+-sensitivity in type II fibers at PRE (by ~0.014 pCa units) and a significantly larger decrease at POST (~0.035 pCa units), indicating that the exercise had increased S‑glutathionylation of fast troponin I. DTT treatment also increased specific force (by ~4%) but only at POST. In contrast, DTT treatment had no effect on either parameter in type I fibers at either PRE or POST. In type I fibers, the decreased Ca2+‑sensitivity was not due to reversible oxidative changes and may have contributed to a decrease in power production during vigorous exercises. In type II fibers, exercise-induced redox changes help counter the decline in Ca2+-sensitivity while causing a small decline in maximum force.Keywords
Funding Information
- Department of Health, Australian Government | National Health and Medical Research Council (1051460, 1051460, 1085331, 1085331, 1085331)
This publication has 53 references indexed in Scilit:
- Transient impairments in single muscle fibre contractile function after prolonged cycling in elite endurance athletesActa Physiologica, 2013
- S‐Glutathionylation of troponin I (fast) increases contractile apparatus Ca2+ sensitivity in fast‐twitch muscle fibres of rats and humansJournal Of Physiology-London, 2012
- Modulation of contractile apparatus Ca2+ sensitivity and disruption of excitation–contraction coupling by S‐nitrosoglutathione in rat muscle fibresJournal Of Physiology-London, 2011
- Acute effects of reactive oxygen and nitrogen species on the contractile function of skeletal muscleJournal Of Physiology-London, 2011
- Exercise-Induced Oxidative Stress: Cellular Mechanisms and Impact on Muscle Force ProductionPhysiological Reviews, 2008
- Hydroxyl radical and glutathione interactions alter calcium sensitivity and maximum force of the contractile apparatus in rat skeletal muscle fibresJournal Of Physiology-London, 2008
- Real‐time measurement of nitric oxide in single mature mouse skeletal muscle fibres during contractionsJournal Of Physiology-London, 2007
- N‐acetylcysteine attenuates the decline in muscle Na+,K+‐pump activity and delays fatigue during prolonged exercise in humansJournal Of Physiology-London, 2006
- Contractile response to low peroxide concentrations: myofibrillar calcium sensitivity as a likely target for redox‐modulation of skeletal muscle functionThe FASEB Journal, 2000
- Free radicals and tissue damage produced by exerciseBiochemical and Biophysical Research Communications, 1982