Myeloid HIF1α Is Involved in the Extent of Orthodontically Induced Tooth Movement
Open Access
- 8 July 2021
- journal article
- research article
- Published by MDPI AG in Biomedicines
- Vol. 9 (7), 796
- https://doi.org/10.3390/biomedicines9070796
Abstract
During orthodontic tooth movement, transcription factor hypoxia-inducible factor 1α (HIF1α) is stabilised in the periodontal ligament. While HIF1α in periodontal ligament fibroblasts can be stabilised by mechanical compression, in macrophages pressure application alone is not sufficient to stabilise HIF1α. The present study was conducted to investigate the role of myeloid HIF1α during orthodontic tooth movement. Orthodontic tooth movement was performed in wildtype and Hif1αΔmyel mice lacking HIF1α expression in myeloid cells. Subsequently, µCT images were obtained to determine periodontal bone loss, extent of orthodontic tooth movement and bone density. RNA was isolated from the periodontal ligament of the control side and the orthodontically treated side, and the expression of genes involved in bone remodelling was investigated. The extent of tooth movement was increased in Hif1αΔmyel mice. This may be due to the lower bone density of the Hif1αΔmyel mice. Deletion of myeloid Hif1α was associated with increased expression of Ctsk and Acp5, while both Rankl and its decoy receptor Opg were increased. HIF1α from myeloid cells thus appears to play a regulatory role in orthodontic tooth movement.Funding Information
- Deutsche Forschungsgemeinschaft (KI2105/1-2)
This publication has 41 references indexed in Scilit:
- Systematic review of ratios between disease /health periodontitis modulators and meta-analysis of their levels in gingival tissue and biological fluidsArchives of Oral Biology, 2021
- Effects of Compressive and Tensile Strain on Macrophages during Simulated Orthodontic Tooth MovementMediators of Inflammation, 2020
- Effects of the Highly COX-2-Selective Analgesic NSAID Etoricoxib on Human Periodontal Ligament Fibroblasts during Compressive Orthodontic Mechanical StrainMediators of Inflammation, 2019
- Effects of ethanol on human periodontal ligament fibroblasts subjected to static compressive forceAlcohol, 2018
- Orthodontic tooth movement: The biology and clinical implicationsThe Kaohsiung Journal of Medical Sciences, 2018
- CD8+ T cells mediate the regenerative PTH effect in hPDL cells via Wnt10b signalingInnate Immunity, 2016
- RANK/RANKL/OPG during orthodontic tooth movementOrthodontics & Craniofacial Research, 2009
- Functions of RANKL/RANK/OPG in bone modeling and remodelingArchives of Biochemistry and Biophysics, 2008
- The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl SandstedtEuropean Journal of Orthodontics, 2005
- Periodontal Ligament Cells Under Mechanical Stress Induce Osteoclastogenesis by Receptor Activator of Nuclear Factor κB Ligand Up-Regulation via Prostaglandin E2 SynthesisJournal of Bone and Mineral Research, 2002