GaN HEMT based biosensor for the detection of breast cancer marker (C-erbB2)
- 19 February 2021
- journal article
- research article
- Published by IOP Publishing in Semiconductor Science and Technology
- Vol. 36 (4), 045018
- https://doi.org/10.1088/1361-6641/abe83e
Abstract
This work reports on the development of a compact GaN HEMTs based biosensor for an easy and early detection of breast cancer biomarker C-erbB2 in the human cell line. The early-stage detection process includes a reaction of antibody-antigen through the cell line culture of a patient. The developed sensing chip has a two-finger gate structure of 125 µm gate width, a gate length of 5 µm. In order to functionalize the gold surface of the fabricated sensor, the sensor chip has been immersed into an optimized 1M aqueous solution of thioglycolic acid at room temperature for 15 Hrs. Various characterizations methods such as I-V, EDS, and FTIR confirm the formation of the Au-S complex. The functionalized sensors have been incubated in phosphate buffer saline solution of 200µg/ml C-erbB-2 monoclonal antibody for 4h. Thereafter, the C-erbB-2 monoclonal antibody conjugated devices are incubated with human cancer cells positive for C-erbB-2 on its cell surface. The biosensor shows a 31% change in drain current for an incubation period of 6 hours. The high-resolution biosensing chip (in terms of high drain current levels of the order of mA) is unaffected by noise and ease the circuit for futuristic point of care diagnostic.Keywords
Funding Information
- Central Electronics Engineering Research Institute (PSC 201)
This publication has 47 references indexed in Scilit:
- The benefits and harms of breast cancer screening: an independent reviewBritish Journal of Cancer, 2013
- AlGaN/GaN high electron mobility transistors for protein–peptide binding affinity studyBiosensors and Bioelectronics, 2013
- Synthesis and characterization of NIR-responsive Aurod@pNIPAAm-PEGMA nanogels as vehicles for delivery of photodynamic therapy agentsNanoscale Research Letters, 2013
- Effect of Three Decades of Screening Mammography on Breast-Cancer IncidenceThe New England Journal of Medicine, 2012
- Quantitative analysis of COOH-terminated alkanethiol SAMs on gold nanoparticle surfacesAdvances in Natural Sciences: Nanoscience and Nanotechnology, 2012
- Graphene‐Encapsulated Nanoparticle‐Based Biosensor for the Selective Detection of Cancer BiomarkersAdvanced Materials, 2011
- Long-term stability study of botulinum toxin detection with AlGaN/GaN high electron mobility transistor based sensorsSensors and Actuators B: Chemical, 2010
- c-erbB-2 sensing using AlGaN∕GaN high electron mobility transistors for breast cancer detectionApplied Physics Letters, 2008
- Polarization effects in nitride semiconductor device structures and performance of modulation doped field effect transistorsSolid-State Electronics, 1999
- RADIOIMMUNOASSAY OF SERUM CREATINE KINASE-BB AS A TUMOUR MARKER IN BREAST CANCERThe Lancet, 1980