Application and Perspectives of MALDI–TOF Mass Spectrometry in Clinical Microbiology Laboratories
Open Access
- 20 July 2021
- journal article
- review article
- Published by MDPI AG in Microorganisms
- Vol. 9 (7), 1539
- https://doi.org/10.3390/microorganisms9071539
Abstract
Early diagnosis of severe infections requires of a rapid and reliable diagnosis to initiate appropriate treatment, while avoiding unnecessary antimicrobial use and reducing associated morbidities and healthcare costs. It is a fact that conventional methods usually require more than 24–48 h to culture and profile bacterial species. Mass spectrometry (MS) is an analytical technique that has emerged as a powerful tool in clinical microbiology for identifying peptides and proteins, which makes it a promising tool for microbial identification. Matrix assisted laser desorption ionization–time of flight MS (MALDI–TOF MS) offers a cost- and time-effective alternative to conventional methods, such as bacterial culture and even 16S rRNA gene sequencing, for identifying viruses, bacteria and fungi and detecting virulence factors and mechanisms of resistance. This review provides an overview of the potential applications and perspectives of MS in clinical microbiology laboratories and proposes its use as a first-line method for microbial identification and diagnosis.Keywords
This publication has 136 references indexed in Scilit:
- Multitask learning for host–pathogen protein interactionsBioinformatics, 2013
- Monitoring the Inflammatory Response to Infection through the Integration of MALDI IMS and MRICell Host & Microbe, 2012
- Novel, Improved Sample Preparation for Rapid, Direct Identification from Positive Blood Cultures Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass SpectrometryThe Journal of Molecular Diagnostics, 2011
- Mutational Analyses of Open Reading Frames within the vraSR Operon and Their Roles in the Cell Wall Stress Response of Staphylococcus aureusAntimicrobial Agents and Chemotherapy, 2011
- From Whole-body Sections Down to Cellular Level, Multiscale Imaging of Phospholipids by MALDI Mass SpectrometryMolecular & Cellular Proteomics, 2011
- Cytoscape 2.8: new features for data integration and network visualizationBioinformatics, 2010
- Analysis of metabolomic PCA data using tree diagramsAnalytical Biochemistry, 2010
- Rapid Classification and Identification of Salmonellae at the Species and Subspecies Levels by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass SpectrometryApplied and Environmental Microbiology, 2008
- BioGRID: a general repository for interaction datasetsNucleic Acids Research, 2006
- Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction NetworksGenome Research, 2003