Retracted: Propofol exerts neuroprotective functions by down‐regulating microRNA‐19a in glutamic acid‐induced PC12 cells
- 8 January 2020
- journal article
- retracted article
- Published by Wiley in BioFactors
- Vol. 46 (6), 934-942
- https://doi.org/10.1002/biof.1607
Abstract
Background Propofol, a kind of intravenous sedative drug, is certified that exerts anti‐inflammation and antitumor functions. However, the influence of propofol in cerebral injury and the corresponding mechanism remains unexplained, that our article focuses on. Methods PC12 cells were treated with propofol and exposed in glutamic acid (Glu) solutions. Cell viability, apoptotic potential, apoptosis‐related and autophagy‐linked proteins were tested via CCK‐8, flow cytometry, and western blot assays. Reverse transcription‐quantitative real‐time PCR was utilized to test miR‐19a expression in Glu‐stimulated cells. Next, miR‐19a mimic transfection was used to assess the effects of miR‐19a on cell apoptosis and autophagy in Glu or propofol treated cells. Finally, western blot was performed to test AMPK and mTOR pathways. Results Glu exposure promoted cell apoptosis and autophagy of PC12 cells, while propofol attenuated cell apoptosis and autophagy triggered by Glu. Additionally, propofol decreased the miR‐19a expression in Glu‐stimulated PC12 cells. Meanwhile, over‐expression of miR‐19a reversed the effects of propofol on Glu‐induced cell apoptosis and autophagy. Moreover, propofol potentiated AMPK and mTOR pathways in Glu‐stimulated PC12 cells via impeding miR‐19a expression. Conclusions These finding revealed that propofol relieved Glu‐triggered apoptosis and autophagy of PC12, and activated AMPK and mTOR pathways by suppressing miR‐19a expression.Keywords
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