Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model
Open Access
- 1 March 2021
- Vol. 26 (5), 1339
- https://doi.org/10.3390/molecules26051339
Abstract
Collagenated porcine-derived bone graft materials exhibit osteoconductive properties and the development of different formulations intends to enhance bone regeneration. This study aims to evaluate bone healing in a rabbit cancellous bone defect in response to grafting with different physicochemical forms of heterologous porcine bone. Twenty-six adult male New Zealand White rabbits received two critical size femoral bone defects per animal (n = 52), each randomly assigned to one of the five tested materials (Apatos, Gen-Os, mp3, Putty, and Gel 40). Animals were sacrificed at 15- and 30-days post-surgery. Qualitative and quantitative (new bone, particle and connective tissue percentages) histological analyses were performed. Histomorphometry showed statistically significant differences in all evaluated parameters between mp3 and both Putty and Gel 40 groups, regardless of the timepoint (p < 0.05). Moreover, statistical differences were observed between Apatos and both Putty (p = 0.014) and Gel 40 (p = 0.007) groups, at 30 days, in regard to particle percentage. Within each group, regarding new bone formation, mp3 showed significant differences (p = 0.028) between 15 (40.93 ± 3.49%) and 30 (52.49 ± 11.04%) days. Additionally, intragroup analysis concerning the percentage of particles revealed a significant reduction in particle occupied area from 15 to 30 days in mp3 and Gen-Os groups (p = 0.009). All mp3, Gen-Os and Apatos exhibited promising results in terms of new bone formation, thus presenting suitable alternatives to be used in bone regeneration.Funding Information
- Fundação para a Ciência e a Tecnologia (04033/2020)
This publication has 68 references indexed in Scilit:
- Ultrastructural study by backscattered electron imaging and elemental microanalysis of bone‐to‐biomaterial interface and mineral degradation of porcine xenografts used in maxillary sinus floor elevationClinical Oral Implants Research, 2012
- Rabbit as an animal model for experimental researchDental Research Journal, 2012
- Issues in modern bone histomorphometryBone, 2011
- Bone regeneration: current concepts and future directionsBMC Medicine, 2011
- Evaluation of an anorganic bovine‐derived mineral with P‐15 hydrogel bone graft: preliminary study in a rabbit cranial bone modelClinical Oral Implants Research, 2011
- Retracted: Experimental Model of Bone Response to Collagenized Xenografts of Porcine Origin (OsteoBiol® mp3): A Radiological and Histomorphometric StudyClinical Implant Dentistry and Related Research, 2011
- Animal research: Reporting in vivo experiments: The ARRIVE guidelinesBritish Journal of Pharmacology, 2010
- Comparison of morphological effects of PGE2 and TGFβ on osteoclastogenesis induced by RANKL in mouse bone marrow cell culturesCell and tissue research, 2007
- Bone Grafts and SubstitutesJournal of Long-Term Effects of Medical Implants, 2006
- Evaluation of autogenic, xenogeneic and alloplastic materials used for cranioplastyCritical Reviews in Neurosurgery, 1997