Longitudinal optical coherence tomography to visualize the in vivo response of middle ear biofilms to antibiotic therapy
Open Access
- 4 March 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-13
- https://doi.org/10.1038/s41598-021-84543-9
Abstract
Studying the impact of antibiotic treatment on otitis media (OM), the leading cause of primary care office visits during childhood, is critical to develop appropriate treatment strategies. Tracking dynamic middle ear conditions during antibiotic treatment is not readily applicable in patients, due to the limited diagnostic techniques available to detect the smaller amount and variation of middle ear effusion (MEE) and middle ear bacterial biofilm, responsible for chronic and recurrent OM. To overcome these challenges, a handheld optical coherence tomography (OCT) system has been developed to monitor in vivo response of biofilms and MEEs in the OM-induced chinchilla model, the standard model for human OM. As a result, the formation of MEE as well as biofilm adherent to the tympanic membrane (TM) was longitudinally assessed as OM developed. Various types of MEEs and biofilms in the chinchilla model were identified, which showed comparable features as those in humans. Furthermore, the effect of antibiotics on the biofilm as well as the amount and type of MEEs was investigated with low-dose and high-dose treatment (ceftriaxone). The capability of OCT to non-invasively track and examine middle ear conditions is highly beneficial for therapeutic OM studies and will lead to improved management of OM in patients.Funding Information
- National Institute for Biomedical Imaging and Bioengineering at the National Institutes of Health (R01 EB013723)
- McGinnis Medical Innovation Fellowship
This publication has 43 references indexed in Scilit:
- The Diagnosis and Management of Acute Otitis MediaPEDIATRICS, 2013
- Noninvasive in vivo optical detection of biofilm in the human middle earProceedings of the National Academy of Sciences of the United States of America, 2012
- Extending the chinchilla middle ear epithelial model for mucin gene investigationInternational Journal of Pediatric Otorhinolaryngology, 2010
- Chinchilla as a robust, reproducible and polymicrobial model of otitis media and its preventionExpert Review of Vaccines, 2009
- Clinical and research applications of anterior segment optical coherence tomography – a reviewClinical & Experimental Ophthalmology, 2009
- Bacterial Biofilms in Otitis MediaThe Pediatric Infectious Disease Journal, 2007
- Direct Detection of Bacterial Biofilms on the Middle-Ear Mucosa of Children With Chronic Otitis MediaJAMA, 2006
- Genomic Sequence of an Otitis Media Isolate of Nontypeable Haemophilus influenzae : Comparative Study with H. influenzae Serotype d, Strain KW20Journal of Bacteriology, 2005
- Outpatient antibiotic use in Europe and association with resistance: a cross-national database studyThe Lancet, 2005
- Complete Genome Sequence of a Virulent Isolate of Streptococcus pneumoniaeScience, 2001