Electrical properties characterization of single yeast cells by dielectrophoretic motion and electro-rotation
- 6 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biomedical Microdevices
- Vol. 23 (1), 1-9
- https://doi.org/10.1007/s10544-021-00550-7
Abstract
The electrical parameters of single cells are label-free and intrinsic properties that can reflect the physiological characteristics. In recent years, many measurement methods based on impedance spectroscopy and rotation spectrum analysis have been developed. However, most of these works need to measure the response at whole frequency range to obtain DEP spectra and estimate the electrical parameters by fitting method, which are time-consuming and limit the measurement throughput. Therefore, improving the measurement throughput for single cells is an essential problem to be solved addressed. In this paper we present a microfluidic chip that combines dielectrophoretic motion and electro-rotation technology for single-cell electrical properties characterization. Since the movement and rotation speed of single cell in mediums are related to the electrical parameters of itself, electric signals and medium, the electrical properties can be obtained by measuring and analyzing the movement trajectory and rotation speed of the cell. Numerical simulations were performed to analyze the electric field distribution of the chip under different signal configurations, which predict the movement trajectory and rotation state, and determine the values of electric field on the cells. Based on the simulation results, cell focusing, dielectrophoretic motion and electro-rotation were successfully realized. By analyzing the movement trajectory and rotation speed, the conductivity of wall and the permittivity of membrane of yeast cells were characterized. The measurement method avoids the time-consuming of the traditional rotational spectra method, and can realize rapid and efficiency and single-cell electrical characterization.Keywords
Funding Information
- Fundamental Research Funds for the Central Universities (JZ2019HGBZ0165)
This publication has 36 references indexed in Scilit:
- An electrorotation technique for measuring the dielectric properties of cells with simultaneous use of negative quadrupolar dielectrophoresis and electrorotationThe Analyst, 2013
- Simultaneous On-Chip DC Dielectrophoretic Cell Separation and Quantitative Separation Performance CharacterizationAnalytical Chemistry, 2012
- Real-time monitoring of cell viability using direct electrical measurement with a patch-clamp microchipBiomedical Microdevices, 2011
- Cell rotation using optoelectronic tweezersBiomicrofluidics, 2010
- A unified approach to dielectric single cell analysis: Impedance and dielectrophoretic force spectroscopyLab on a Chip, 2010
- Contactless dielectrophoresis: a new technique for cell manipulationBiomedical Microdevices, 2009
- Selective trapping of live and dead mammalian cells using insulator-based dielectrophoresis within open-top microstructuresBiomedical Microdevices, 2008
- Mapping normal and cancer cell signalling networks: towards single-cell proteomicsNature Reviews Cancer, 2006
- Dielectric behavior of wild-type yeast and vacuole-deficient mutant over a frequency range of 10 kHz to 10 GHzBiophysical Journal, 1996
- Computer-aided analyses of electric fields used in electrorotation studiesJournal of Physics D: Applied Physics, 1994