Effects of Copper Oxide Nanoparticles on the Growth of Rice (Oryza Sativa L.) Seedlings and the Relevant Physiological Responses
Open Access
- 15 February 2020
- journal article
- research article
- Published by MDPI AG in International Journal of Environmental Research and Public Health
- Vol. 17 (4), 1260
- https://doi.org/10.3390/ijerph17041260
Abstract
Rice (Oryza sativa L.), a major staple food for billions of people, was assessed for its phytotoxicity of copper oxide nanoparticle (CuO NPs, size < 50 nm). Under hydroponic condition, seven days of exposure to 62.5, 125, and 250 mg/L CuO NPs significantly suppressed the growth rate of rice seedlings compared to both the control and the treatment of supernatant from 250 mg/L CuO NP suspensions. In addition, physiological indexes associated with antioxidants, including membrane damage and antioxidant enzyme activity, were also detected. Treatment with 250 mg/L CuO NPs significantly increased malondialdehyde (MDA) content and electrical conductivity of rice shoots by 83.4% and 67.0%, respectively. The activity of both catalase and superoxide dismutase decreased in rice leaves treated with CuO NPs at the concentration of 250 mg/L, while the activity of the superoxide dismutase significantly increased by 1.66 times in rice roots exposed to 125 mg/L CuO NPs. The chlorophyll, including chlorophyll a and chlorophyll b, and carotenoid content in rice leaves decreased with CuO NP exposure. Finally, to explain potential molecular mechanisms of chlorophyll variations, the expression of four related genes, namely, Magnesium chelatase D subunit, Chlorophyll synthase, Magnesium-protoporphyrin IX methyltransferase, and Chlorophyllide a oxygenase, were quantified by qRT-PCR. Overall, CuO NPs, especially at 250 mg/L concentration, could affect the growth and development of rice seedlings, probably through oxidative damage and disturbance of chlorophyll and carotenoid synthesis.Keywords
Funding Information
- National Natural Science Foundation of China (31570452)
- Ministry of Science and Technology (2014DFA31740)
This publication has 53 references indexed in Scilit:
- Stress Response and Tolerance of Zea mays to CeO2 Nanoparticles: Cross Talk among H2O2, Heat Shock Protein, and Lipid PeroxidationACS Nano, 2012
- Cytotoxicity and ion release of alloy nanoparticlesJournal of Nanoparticle Research, 2012
- Cytotoxic Origin of Copper(II) Oxide Nanoparticles: Comparative Studies with Micron-Sized Particles, Leachate, and Metal SaltsACS Nano, 2011
- To duckweeds (Landoltia punctata), nanoparticulate copper oxide is more inhibitory than the soluble copper in the bulk solutionEnvironmental Pollution, 2011
- Understanding Oxidative Stress and Antioxidant Functions to Enhance PhotosynthesisPlant Physiology, 2010
- CuO Nanoparticles Catalyzed C−N, C−O, and C−S Cross-Coupling Reactions: Scope and MechanismThe Journal of Organic Chemistry, 2009
- Copper toxicity in Withania somnifera: Growth and antioxidant enzymes responses of in vitro grown plantsEnvironmental and Experimental Botany, 2008
- Phytotoxicity of nanoparticles: Inhibition of seed germination and root growthEnvironmental Pollution, 2007
- Cu nanoparticles derived from CuO electrodes in lithium cellsNanotechnology, 2005
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001