Preventive Effects of Neuroprotective Agents in a Neonatal Rat of Photothrombotic Stroke Model
Open Access
- 24 May 2020
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (10), 3703
- https://doi.org/10.3390/ijms21103703
Abstract
Neonatal ischemic stroke has a higher incidence than childhood stroke. Seizures are the first sign for the need for clinical assessment in neonates, but many questions remain regarding treatments and follow-up modalities. In the absence of a known pathophysiological mechanism, only supportive care is currently provided. Stroke-induced microglia activation and neuroinflammation are believed to play a central role in the pathological progression of neonatal ischemic stroke. We induced a photothrombotic infarction with Rose Bengal in neonatal rats to investigate the effects of pre- and post-treatment with Aspirin (ASA), Clopidogrel (Clop), and Coenzyme Q10 (CoQ10), which are known for their neuroprotective effects in adult stroke. Pre-stroke medication ameliorates cerebral ischemic injury and reduces infarct volume by reducing microglia activation, cellular reactive oxygen species (ROS) production, and cytokine release. Post-stroke administration of ASA, Clop, and CoQ10 increased motor function and reduced the volume of infarction, and the statistical evidence was stronger than that seen in the pre-stroke treatment. In this study, we demonstrated that ASA, Clop, and CoQ10 treatment before and after the stroke reduced the scope of stroke lesions and increased behavioral activity. It suggests that ASA, Clop, and CoQ10 medication could significantly have neuroprotective effects in the neonates who have suffered strokes.Funding Information
- National Research Foundation of Korea (2020R1C1C1007488, 2019R1A2C2004884, 2018R1D1A1B07045664, 2015R1C1A1A01052351)
This publication has 50 references indexed in Scilit:
- Management of Stroke in Neonates and Children: A Scientific Statement From the American Heart Association/American Stroke AssociationStroke, 2019
- 2018 Guidelines for the Early Management of Patients With Acute Ischemic Stroke: A Guideline for Healthcare Professionals From the American Heart Association/American Stroke AssociationStroke, 2018
- Sources of tissue factor that contribute to thrombosis after rupture of an atherosclerotic plaqueThrombosis Research, 2012
- Antithrombotic Therapy in Neonates and Children: Antithrombotic Therapy and Prevention of Thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice GuidelinesSocial psychiatry. Sozialpsychiatrie. Psychiatrie sociale, 2012
- Impact of intracranial blood-flow redistribution on stroke size during ischemia–reperfusion in 7-day-old ratsJournal of Neuroscience Methods, 2011
- Arterial ischemic stroke risk factors: The international pediatric stroke studyAnnals of Neurology, 2011
- Management of Stroke in Infants and ChildrenStroke, 2008
- Risk of Recurrent Childhood Arterial Ischemic Stroke in a Population-Based Cohort: The Importance of Cerebrovascular ImagingPEDIATRICS, 2007
- Induction of Reproducible Focal Ischemic Lesions in Neonatal Mice by PhotothrombosisDevelopmental Neuroscience, 2005
- Long-term risk of recurrent stroke after a first-ever stroke. The Oxfordshire Community Stroke Project.Stroke, 1994