Grafting Delays Watermel on Fruit Ripening by Altering Gene Expression of ABA Centric Phytohormone Signaling
Open Access
- 25 February 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Plant Science
Abstract
Grafting cultivation is implemented world-wide mainly to resist abiotic and biotic stress, and an effective method to improve watermelon production. However, grafting may affect fruit development and quality. In our experiment, pumpkin grafted (PG) watermelon fruits developed slower and the ripening period was extended compared to self grafted (SG) fruits. We found the concentrations of Abscisic acid (ABA) among endogenous phytohormones were dramatically reduced by pumpkin grafting. In order to understand these changes at the gene expression level, we performed a comprehensive analysis of the fruit flesh transcriptomes between PG and SG during fruit development and ripening. Total of 1675 and 4102 deferentially expressed genes (DEGs) were identified between PG and SG. Further functional enrichment analysis revealed that these DEGs were associated with carbohydrate biosynthesis, phytohormone signaling transmission and cell wall metabolism categories. ABA centric phytohormones signaling and fruit quality related genes including ABA receptor, PP2C proteins, AP2-EREBP transcription factors, sucrose transporter and carotenoid isomerase were co-expressed with fruit ripening. These results provide the valuable resource for understanding the mechanism of pumpkin grafting effect on watermelon fruit ripening and quality development.Keywords
Funding Information
- National Key Research and Development Program of China
- Agriculture Research System of China
- Ministry of Science and Technology of the People’s Republic of China (2016YFD0101007, 2015BAD02B02)
- National Natural Science Foundation of China
This publication has 33 references indexed in Scilit:
- The role of abscisic acid in regulating cucumber fruit development and ripening and its transcriptional regulationPlant Physiology and Biochemistry, 2013
- The Citrus ABA signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydrationJournal of Experimental Botany, 2012
- Impact of Grafting on Watermelon Growth, Fruit Yield and QualityJournal of Fruit and Ornamental Plant Research, 2012
- Abscisic Acid Plays an Important Role in the Regulation of Strawberry Fruit RipeningPlant Physiology, 2011
- Differential expression analysis for sequence count dataGenome Biology, 2010
- TopHat: discovering splice junctions with RNA-SeqBioinformatics, 2009
- Ultrafast and memory-efficient alignment of short DNA sequences to the human genomeGenome Biology, 2009
- A General Framework for Weighted Gene Co-Expression Network AnalysisStatistical Applications in Genetics and Molecular Biology, 2005
- GO::TermFinder—open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genesBioinformatics, 2004
- Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray ExperimentsStatistical Applications in Genetics and Molecular Biology, 2004