Adipose‑derived mesenchymal stem cells inhibit cell proliferation and migration and suppress extracellular matrix synthesis in hypertrophic‑scar and keloid fibroblasts
Open Access
- 13 December 2020
- journal article
- research article
- Published by Spandidos Publications in Experimental and Therapeutic Medicine
- Vol. 21 (2), 1
- https://doi.org/10.3892/etm.2020.9571
Abstract
Pathological scars occur during skin wound healing, and the use of adipose‑derived stem cells (ADSCs) is one of the various treatments. The present study aimed to investigate the in vitro effects of ADSCs on the biological properties of hypertrophic scar fibroblasts (HSFs) and keloid fibroblasts (KFs), such as proliferation, migration, and the synthesis of extracellular matrix proteins. Transwell chambers were used to establish a co‑culture system of ADSCs with normal skin fibroblasts (NFs), HSFs or KFs. The effect of ADSCs on the proliferation of fibroblasts was evaluated by CCK8 measurement, while the migration ability of fibroblasts was assessed using cell scratch assay. The expression of extracellular matrix proteins was measured by immunoblotting. Co‑culture of NFs with ADSCs did not affect cell proliferation and migration, nor the expression of extracellular matrix proteins [collagen‑I, collagen‑III, fibronectin (FN) and α‑smooth muscle actin (α‑SMA)] in NFs. However, as with the inhibitor SB431542, ADSCs significantly inhibited cell proliferation and migration and the expression of extracellular matrix proteins (collagen‑I, collagen‑III, FN and α‑SMA), but also suppressed the protein expression of transforming growth factor β1 (TGF‑β1), phosphorylated (p‑) mothers against decapentaplegic homolog (Smad) 2, p‑Smad3 and Smad7 in HSFs and KFs. The results show that ADSCs inhibited cell proliferation and migration and the expression of extracellular matrix proteins in HSCs and KFs in vitro, possibly through inhibition of the TGF‑β1/Smad pathway.Keywords
This publication has 50 references indexed in Scilit:
- The Relative Contribution of Paracine Effect versus Direct Differentiation on Adipose-Derived Stem Cell Transplantation Mediated Cardiac RepairPLOS ONE, 2013
- Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4+FOXP3+ Cells and Increases IL-10 SecretionJournal of Biomedicine and Biotechnology, 2012
- Transplantation of Mesenchymal Stem Cells Promotes an Alternative Pathway of Macrophage Activation and Functional Recovery after Spinal Cord InjuryJournal of Neurotrauma, 2012
- Transforming growth factor-beta 1 in adipose derived stem cells conditioned medium is a dominant paracrine mediator determines hyaluronic acid and collagen expression profileCytotechnology, 2011
- The Modified Patient and Observer Scar Assessment Scale: A Novel Approach to Defining Pathologic and Nonpathologic ScarringPlastic and Reconstructive Surgery, 2011
- Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T cell proliferation and collagen-induced arthritisArthritis Research & Therapy, 2010
- Mesenchymal stem cells induce dermal fibroblast responses to injuryExperimental Cell Research, 2009
- Human Umbilical Cord Mesenchymal Stem Cells Reduce Fibrosis of Bleomycin-Induced Lung InjuryThe American Journal of Pathology, 2009
- Immune regulation by mesenchymal stem cells: two sides to the coinTissue Antigens, 2006
- Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering, 2001