Self-Regeneration and Self-Healing in DNA Origami Nanostructures
Open Access
- 23 February 2021
- journal article
- research article
- Published by Wiley in Angewandte Chemie-International Edition
- Vol. 60 (9), 4931-4938
- https://doi.org/10.1002/anie.202012986
Abstract
DNA nanotechnology and advances in the DNA origami technique have enabled facile design and synthesis of complex and functional nanostructures. Molecular devices are, however, prone to rapid functional and structural degradation due to the high proportion of surface atoms at the nanoscale and due to complex working environments. Besides stabilizing mechanisms, approaches for the self-repair of functional molecular devices are desirable. Here we exploit the self-assembly and reconfigurability of DNA origami nanostructures to induce the self-repair of defects of photoinduced and enzymatic damage. We provide examples of repair in DNA nanostructures showing the difference between unspecific self-regeneration and damage specific self-healing mechanisms. Using DNA origami nanorulers studied by atomic force and superresolution DNA PAINT microscopy, quantitative preservation of fluorescence properties is demonstrated with direct potential for improving nanoscale calibration samples.Funding Information
- Horizon 2020 Framework Programme (737089, 840741)
- Alexander von Humboldt-Stiftung
- Deutsche Forschungsgemeinschaft (86/1904-1 FUGG, 201269156 - SFB 1032, EXC2089/1-390776260)
This publication has 52 references indexed in Scilit:
- A method to study in vivo stability of DNA nanostructuresMethods, 2013
- Stability of DNA Origami Nanoarrays in Cell LysateNano Letters, 2011
- Endogenous versus Exogenous DNA Adducts: Their Role in Carcinogenesis, Epidemiology, and Risk AssessmentToxicological Sciences, 2010
- Single-Molecule Kinetics and Super-Resolution Microscopy by Fluorescence Imaging of Transient Binding on DNA OrigamiNano Letters, 2010
- Self-healing at the nanoscaleNanoscale, 2009
- Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopyProceedings of the National Academy of Sciences of the United States of America, 2009
- Self-assembly of a nanoscale DNA box with a controllable lidNature, 2009
- On the Mechanism of Trolox as Antiblinking and Antibleaching ReagentJournal of the American Chemical Society, 2009
- A practical guide to single-molecule FRETNature Methods, 2008
- Folding DNA to create nanoscale shapes and patternsNature, 2006