Development and screening of byproduct for its secondary metabolites, antioxidant and anti-diabetic potential from anthracnose-infected fruits of pomegranate: a sustainable approach
- 15 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in 3 Biotech
- Vol. 11 (2), 1-11
- https://doi.org/10.1007/s13205-020-02629-z
Abstract
The main focus of the present study was to analyze the antioxidant and anti-diabetic potential of fermentative byproduct, developed from anthracnose-infected pomegranate fruits. The analysis of fermented juice showed a reduction in total phenolic content, total flavanoid content, anthocyanins, and antioxidant potential over the time in 6 months as compared to fresh juice, while total protein and alcohol percent (11%) were increased. Measurements of antioxidant activity by DPPH, ABTS, superoxide radical scavenging activity, and reducing power assays were highly correlated to total phenolic content, with corresponding R2 values as rDPPH = 0.88, rABTS = 0.90, rSRS = 0.67, rRPA = 0.80. High-performance liquid chromatography clearly revealed that the increment of antioxidant activity is associated with the release of gallic acid, vanillin, and ferulic acid. LC–MS analysis identified 1263 metabolites in fresh juice, 1580 metabolites after 1 month of fermentation, and 1063 metabolites after 6 months of fermentation. Most of the detected metabolites are linked with antioxidant, anti-diabetic, phenolics, flavanoids, cardiac glycosides, anticancer, and anti-vomiting activity. Mainly, naphthofluorescein, CAY10599, CAY10506, aminofluoropropionic acid, and 8-azaadenosine anti-diabetic compounds were found in fresh juice and fermented juice. Administration of fresh juice and fermented juice for 1 month helped in the reduction of blood plasma glucose level from 112.6 to 94.73 mg/dL before food and 142.43 to 133.20 mg/dL after food as compared to prescribed medicine. The sensory attributes of fermented juice were well appreciated for taste, after taste, and flavor. Further research is necessary to improve the quality and stability of metabolites during storage.Keywords
This publication has 38 references indexed in Scilit:
- Evolution of Analysis of Polyhenols from Grapes, Wines, and ExtractsMolecules, 2013
- Evaluation of phenolic contents and antioxidant activity of various solvent extracts of Sonchus asper (L.) HillChemistry Central Journal, 2012
- Analysis of volatile compounds of mango wineFood Chemistry, 2011
- Effect of probiotication on antioxidant and antibacterial activities of pomegranate juices from sour and sweet cultivarsNatural Product Research, 2011
- Identification and quantification of phenolic compounds and their effects on antioxidant activity in pomegranate juices of eight Iranian cultivarsFood Chemistry, 2009
- Changes in chemical constituents during the maturation and ripening of two commercially important pomegranate accessionsFood Chemistry, 2009
- Perspectives for Cancer Prevention With Natural CompoundsJournal of Clinical Oncology, 2009
- Antioxidant Activity, Polyphenol Content, and Related Compounds in Different Fruit Juices and Homogenates Prepared from 29 Different Pomegranate AccessionsJournal of Agricultural and Food Chemistry, 2007
- Analytical characterization of the aroma of Tinta Negra Mole red wine: Identification of the main odorants compoundsAnalytica Chimica Acta, 2006
- Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stagesRemote Sensing of Environment, 2002