Genomics-driven discovery of a new cyclodepsipeptide from the guanophilic fungusAmphichorda guana
- 12 February 2021
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Organic & Biomolecular Chemistry
- Vol. 19 (9), 1960-1964
- https://doi.org/10.1039/d1ob00100k
Abstract
Two potential non-ribosomal peptide synthetases (NRPSs) were identified in the genome of a guanophilic fungus Amphichorda guana by bioinformatics analysis and gene knockout experiments. Liquid chromatography coupled with mass spectrometry (LC-MS) guided isolation led to the discovery of a new cyclodepsipeptide isaridin H (1) and seven known analogs, desmethylisaridin E (2), isaridin E (3), isariin A (4), iso-isariin B (5), iso-isariin D (6), isariin E (7), and nodupetide (8). The absolute configuration of isaridin H (1) was achieved by Marfey's method. Isaridin H (1) showed significant antifungal activity against Botrytis cinerea and Alternaria solani.Funding Information
- National Key Research and Development Program of China (2018YFA0901901)
- National Natural Science Foundation of China (81872771)
- Key Research Program of Frontier Science, Chinese Academy of Sciences (ZDBS-LY-SM016)
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