Semen parameters on the intracytoplasmic sperm injection day: Predictive values and cutoff thresholds of success
Open Access
- 1 March 2021
- journal article
- research article
- Published by The Korean Society for Reproductive Medicine in Clinical and Experimental Reproductive Medicine
- Vol. 48 (1), 61-68
- https://doi.org/10.5653/cerm.2020.03965
Abstract
Objective: This study was conducted to investigate the relationship of semen parameters in samples used for intracytoplasmic sperm injection (ICSI) with fertilization and pregnancy rates in infertile couples. Methods: In this prospective study of Infertile couples with male factor infertility that had undergone ICSI, fractions of the same semen samples obtained for microinjection (to ensure the best predictability) were evaluated to determine the semen parameters and sperm DNA fragmentation index (DFI) on the day of oocyte recovery. Results: In total, 120 couples completed the study and were subdivided into fertilized (n=87) and non-fertilized couples (n=33). The fertilized couples were further classified into pregnant (n=48) and non-pregnant (n=39) couples. Compared to non-fertilized and non-pregnant couples, fertilized and pregnant couples showed statistically significantly higher sperm viability and percentage of normal sperm morphology, as well as significantly lower sperm DFI values. A receiver operating characteristic curve analysis of data from the 120 ICSI cycles showed that sperm viability, normal sperm morphology percentages, and sperm DFI were significant prognostic indicators of fertilization at cutoff values of 40%, 7%, and 46%, respectively. A sperm DFI of 46% showed sensitivity and specificity of 95% and 90%, respectively, for predicting fertilization, and no clinical pregnancies occurred in couples with a sperm DFI above 46%. Conclusion: Semen parameters from the ICSI day sample, especially sperm viability, normal morphology, and DFI, had an impact on fertilization and pregnancy outcomes in ICSI cycles.Keywords
This publication has 27 references indexed in Scilit:
- The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-analysisReproductive BioMedicine Online, 2015
- Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysisJournal of Assisted Reproduction and Genetics, 2014
- Best Protocol for Controlled Ovarian Hyperstimulation in Assisted Reproductive Technologies: Fact or Opinion?Seminars in Reproductive Medicine, 2014
- Relationship between DNA damage in sperm after ex vivo exposure and abnormal embryo development in the progeny of the three-spined sticklebackReproductive Toxicology, 2013
- The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysisHuman Reproduction, 2012
- Clinical significance of sperm DNA damage in assisted reproduction outcomeHuman Reproduction, 2010
- Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoaAsian Journal of Andrology, 2010
- Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysisHuman Reproduction, 2008
- Combination of Hypoosmotic Swelling/Eosin Y Test for Sperm Membrane Integrity Evaluation: Correlations with Other Sperm Parameters to Predict ICSI CyclesArchives of Andrology, 2007
- Correlation of sperm DNA damage with IVF and ICSI outcomes: A systematic review and meta-analysisJournal of Assisted Reproduction and Genetics, 2006