Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice
- 15 March 2023
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Food & Function
- Vol. 14 (7), 3092-3106
- https://doi.org/10.1039/d2fo04092a
Abstract
Calcium deficiency can lead to osteoporosis. Adequate calcium intake can improve calcium deficiency and prevent osteoporosis. Milk powder is the best source of dietary calcium supplements. Probiotics and prebiotics are considered to be beneficial substances for promoting calcium absorption. In this study, synbiotic milk powder (SMP) was prepared by combining the three, and its calcium supplementation effect and osteogenic activity were evaluated in calcium deficient mice. Through prebiotic screening experiments in vitro, after adding 1.2% iso-malto-oligosaccharide, the number of viable bacteria and the calcium enrichment of Lactobacillus plantarum JJBYG12 increased by 8.15% and 94.53% compared with those of the control group. Long-term calcium deficiency led to a significant reduction in calcium absorption and bone calcium content in mice, accompanied by structural deterioration of bone trabeculae. SMP significantly improved apparent calcium absorption, increased serum calcium and phosphorus levels, and decreased ALP activity and CTX-1 levels. In the meantime, the bone mineral density increased significantly, and the number of bone trabeculae and the proliferation and differentiation of osteoblasts also increased. SMP has good dietary calcium supplementation capacity and bone remodeling ability without significant side effects on major organs. These findings provide insights into using SMP as a dietary calcium source to improve bone health.This publication has 71 references indexed in Scilit:
- Comparing the calcium bioavailability from two types of nano-sized enriched milk using in-vivo assayFood Chemistry, 2017
- Evaluation of safety of modified-Danggui Buxue Tang in rodents:immunological, toxicity and hormonal aspectsJournal of Ethnopharmacology, 2016
- Probiotics (Bifidobacterium longum) Increase Bone Mass Density and UpregulateSparcandBmp-2Genes in Rats with Bone Loss Resulting from OvariectomyBioMed Research International, 2015
- Administration of caseinomacropeptide-enriched extract to mice enhances the calcium content of femur in a low-calcium dietInternational Dairy Journal, 2014
- Combination therapy of Chinese herbal medicine Fructus Ligustri Lucidi with high calcium diet on calcium imbalance induced by ovariectomy in micePharmaceutical Biology, 2014
- Retention of bone strength by feeding of milk and dairy products in ovariectomized rats: involvement of changes in serum levels of 1alpha, 25(OH)2D3 and FGF23The Journal of Nutritional Biochemistry, 2013
- Enzymology and Structure of the GH13_31 Glucan 1,6-α-Glucosidase That Confers Isomaltooligosaccharide Utilization in the Probiotic Lactobacillus acidophilus NCFMJournal of Bacteriology, 2012
- Yacon Flour andBifidobacterium longumModulate Bone Health in RatsJournal of Medicinal Food, 2012
- Antiosteoporotic Effects of Lactobacillus-Fermented Soy Skim Milk on Bone Mineral Density and the Microstructure of Femoral Bone in Ovariectomized MiceJournal of Agricultural and Food Chemistry, 2011
- Fructo-oligosaccharides enhance the mineral absorption and counteract the adverse effects of phytic acid in miceNutrition, 2010