Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
Open Access
- 11 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 12 (1), 1-8
- https://doi.org/10.1038/s41467-021-21238-9
Abstract
The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.This publication has 30 references indexed in Scilit:
- cellSTORM—Cost-effective super-resolution on a cellphone using dSTORMPLOS ONE, 2019
- Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical SensingPharmaceuticals, 2018
- Single-Molecule Plasmon Sensing: Current Status and Future ProspectsACS Sensors, 2017
- Plasmon-enhanced fluorescence spectroscopyChemical Society Reviews, 2017
- Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopyNature Communications, 2017
- Plasmon-Enhanced Fluorescence Biosensors: a ReviewPlasmonics, 2013
- A plasmonic ‘antenna-in-box’ platform for enhanced single-molecule analysis at micromolar concentrationsNature Nanotechnology, 2013
- Nucleic acid amplification: Alternative methods of polymerase chain reactionJournal of Pharmacy And Bioallied Sciences, 2013
- Optical Methods for Single Molecule Detection and AnalysisAnalytical Chemistry, 2012
- Nucleic Acid Isothermal Amplification Technologies—A ReviewNucleosides, Nucleotides and Nucleic Acids, 2008