Gene editing to investigate the role of conceptus factors in the establishment of pregnancy in the pig
- 1 April 2021
- journal article
- review article
- Published by Bioscientifica in Reproduction
- Vol. 161 (4), R79-R88
- https://doi.org/10.1530/rep-20-0604
Abstract
Development of viviparity in mammals required that the placenta evolve as an intermediate interface between the fetus and maternal uterus. In addition to the retention of the fetus and secretion of nutrients to support growth and development to term, it was essential that viviparous species modify or inhibit the maternal immune system from recognizing the semi-allogeneic fetus. Following blastocyst hatching from its zona pellucida, trophoblast differentiation provides the initial communication to the maternal endometrium to regulate maintenance of progesterone production from the corpus luteum and biological pathways in uterine and conceptus development necessary in establishment and maintenance of pregnancy. Many conceptus factors have been proposed to serve in the establishment and maintenance of pregnancy. CRISPR-Cas9 gene editing technology provides a specific and efficient method to generate animal models to perform loss of function studies to investigate the role of specific conceptus factors. The utilization of CRISPR-Cas9 gene editing has provided a direct approach to investigate the specific role of conceptus factors in development and establishment of pregnancy in the pig. This technology has helped address a number of questions concerning peri-implantation development and altered our understanding of maternal recognition and maintenance of pregnancy in the pig.Keywords
This publication has 40 references indexed in Scilit:
- CRISPR-Mediated Adaptive Immune Systems in Bacteria and ArchaeaAnnual Review of Biochemistry, 2013
- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial ImmunityScience, 2012
- Interferon Gamma in Successful Pregnancies1Biology of Reproduction, 2009
- Estradiol-17β, Prostaglandin E2 (PGE2), and the PGE2 Receptor Are Involved in PGE2 Positive Feedback Loop in the Porcine EndometriumEndocrinology, 2009
- Immunological and Inflammatory Functions of the Interleukin-1 FamilyAnnual Review of Immunology, 2009
- Identification of differential gene expression during porcine conceptus rapid trophoblastic elongation and attachment to uterine luminal epitheliumPhysiological Genomics, 2009
- Interferons and Uterine ReceptivitySeminars in Reproductive Medicine, 2009
- Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pigJournal of Endocrinology, 2007
- Mechanisms of type-I- and type-II-interferon-mediated signallingNature Reviews Immunology, 2005
- Ovarian interleukin-1-induced gene expression: privileged genes threshold theoryMedical Hypotheses, 2002