The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O
Open Access
- 25 February 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Veterinary Science
Abstract
Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)–fused capsid proteins of FMDV serotype O by single plasmid in Escherichia coli was achieved with an optimal tandem permutation (VP0–VP3–VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine.Keywords
This publication has 35 references indexed in Scilit:
- Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty CapsidsPLOS ONE, 2013
- Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattleVeterinary Research, 2013
- Development of a Foot-and-Mouth Disease Virus Serotype A Empty Capsid Subunit Vaccine Using Silkworm (Bombyx mori) PupaePLOS ONE, 2012
- Production of FMDV virus-like particles by a SUMO fusion protein approach in Escherichia coliJournal of Biomedical Science, 2009
- Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigsVeterinary Microbiology, 2009
- Development of transgenic alfalfa plants containing the foot and mouth disease virus structural polyprotein gene P1 and its utilization as an experimental immunogenVaccine, 2005
- Foot-and-Mouth DiseaseClinical Microbiology Reviews, 2004
- Further studies on the early protective responses of pigs following immunisation with high potency foot and mouth disease vaccineVaccine, 2002
- Assembly of foot-and-mouth disease virus empty capsids synthesized by a vaccinia virus expression systemJournal of General Virology, 1995
- Evidence for more than one important, neutralizing site on foot-and-mouth disease virusArchiv für die gesamte Virusforschung, 1988