Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome
Open Access
- 5 January 2021
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 220 (2)
- https://doi.org/10.1083/jcb.202009083
Abstract
Centrosomes are composed of a centriolar core surrounded by a pericentriolar material (PCM) matrix that docks microtubule-nucleating γ-tubulin complexes. During mitotic entry, the PCM matrix increases in size and nucleating capacity in a process called centrosome maturation. Polo-like kinase 1 (PLK1) is recruited to centrosomes and phosphorylates PCM matrix proteins to drive their self-assembly, which leads to PCM expansion. Here, we show that in addition to controlling PCM expansion, PLK1 independently controls the generation of binding sites for γ-tubulin complexes on the PCM matrix. Selectively preventing the generation of PLK1-dependent γ-tubulin docking sites led to spindle defects and impaired chromosome segregation without affecting PCM expansion, highlighting the importance of phospho-regulated centrosomal γ-tubulin docking sites in spindle assembly. Inhibiting both γ-tubulin docking and PCM expansion by mutating substrate target sites recapitulated the effects of loss of centrosomal PLK1 on the ability of centrosomes to catalyze spindle assembly.Keywords
Funding Information
- National Institutes of Health (GM074207, T32 CA067754, F32GM125347)
- Japan Society for the Promotion of Science
- Cancer Cell Biology Training Program (T32 CA067754, F32GM125347)
- Ludwig Institute for Cancer Research
This publication has 63 references indexed in Scilit:
- PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosisThe Journal of cell biology, 2011
- Limiting Amounts of Centrosome Material Set Centrosome Size in C. elegans EmbryosCurrent Biology, 2011
- CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complexThe Journal of cell biology, 2010
- Microtubule nucleating γ-TuSC assembles structures with 13-fold microtubule-like symmetryNature, 2010
- Two distinct regions of Mto1 are required for normal microtubule nucleation and efficient association with the γ-tubulin complex in vivoJournal of Cell Science, 2008
- Single-copy insertion of transgenes in Caenorhabditis elegansNature Genetics, 2008
- A Protein Domain-Based Interactome Network for C. elegans Early EmbryogenesisCell, 2008
- CDK5RAP2 Is a Pericentriolar Protein That Functions in Centrosomal Attachment of the γ-Tubulin Ring ComplexMolecular Biology of the Cell, 2008
- Proper Recruitment of γ-Tubulin and D-TACC/Msps to EmbryonicDrosophilaCentrosomes Requires Centrosomin Motif 1Molecular Biology of the Cell, 2007
- Flies without CentriolesCell, 2006