Identification of functional circRNA/miRNA/mRNA regulatory network for exploring prospective therapy strategy of colorectal cancer
- 1 March 2020
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 121 (12), 4785-4797
- https://doi.org/10.1002/jcb.29703
Abstract
Circular RNA (circRNA) has been reported to have great scientific significance and clinical value in multiple cancers including colorectal cancer (CRC). However, the biological function of most circRNAs in CRC is still in its infancy. Herein, we discovered the differential expressed circRNAs (DECs) between CRC tissues and matched adjacent using deep RNA sequencing and further confirmed the DECs expression by combining with another Gene Expression Omnibus dataset. Furthermore, we validated the expression of the top four upregulated circRNAs (hsa_circ_0030632, hsa_circ_0004887, hsa_circ_0001550, and hsa_circ_0001681) in both of paired CRC tissues and CRC cell lines. Then, a circRNA/microRNA/messenger RNA regulatory network was established and the Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed these four circRNAs participated in various biological processed including apoptotic process and multiple metabolic processes. Moreover, based on the regulatory network, three bioactive compounds (pergolide, pivampicillin, and methylergometrine) for the treatment of CRC were also found. In conclusion, this study improved our understanding of circRNAs and may also facilitate the finding of promising targets and biomarkers in CRC.Funding Information
- National Natural Science Foundation of China (31828005, 81501346, 81601370, 81603149, 81673475, 81872905)
This publication has 45 references indexed in Scilit:
- microRNA-139-5p exerts tumor suppressor function by targeting NOTCH1 in colorectal cancerMolecular Cancer, 2014
- starBase v2.0: decoding miRNA-ceRNA, miRNA-ncRNA and protein–RNA interaction networks from large-scale CLIP-Seq dataNucleic Acids Research, 2013
- Changes in colorectal cancer incidence rates in young and older adults in the United States: what does it tell us about screening.Cancer Causes & Control, 2013
- From tumor cell metabolism to tumor immune escapeThe International Journal of Biochemistry & Cell Biology, 2013
- Circular RNAs are abundant, conserved, and associated with ALU repeatsRNA, 2012
- Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell TypesPLOS ONE, 2012
- Hallmarks of Cancer: The Next GenerationCell, 2011
- Increase in Incidence of Colorectal Cancer Among Young Men and Women in the United StatesCancer Epidemiology, Biomarkers & Prevention, 2009
- The Connectivity Map: Using Gene-Expression Signatures to Connect Small Molecules, Genes, and DiseaseScience, 2006
- Cytochrome C-Mediated ApoptosisAnnual Review of Biochemistry, 2004