Digital counting of single semiconducting polymer nanoparticles for the detection of alkaline phosphatase
- 1 February 2021
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Nanoscale
- Vol. 13 (9), 4946-4955
- https://doi.org/10.1039/d0nr09232k
Abstract
Alkaline phosphatase (ALP) as a necessary hydrolase in phosphate metabolism is closely related to various diseases. Ultrasensitive detection of ALP with a convenient and sensitive method is of fundamental importance. In this work, a fluorescence resonance energy transfer (FRET)-based single-particle enumeration (SPE) method is proposed for the quantitative analysis of ALP. This strategy is based on the effective fluorescence suppression by a polydopamine (PDA) shell on the surface of semiconducting polymer nanoparticles (SPNs). PDA with broadband absorption in the UV-vis region can serve as an excellent quencher for SPNs. However, ascorbic acid (AA), the product of the hydrolysis of 2-phosphate-L-ascorbic acid trisodium salt (AAP) in the presence of ALP, can effectively inhibit the self-polymerization of dopamine (DA) to form a PDA layer. Therefore, ALP can be accurately quantified by counting the concentration-related fluorescent particles in the fluorescence image. A linear range from 0.031 to 12.4 μU mL−1 and a limit-of-detection (LOD) of 0.01 μU mL−1 for ALP determination are achieved. The spiked recoveries for ALP determination in a human serum sample are between 90% and 108% with RSD less than 3.1%. In summary, this convenient and sensitive approach proposed here provides promising prospects for ALP detection in a complex biological matrix.Keywords
Funding Information
- National Natural Science Foundation of China (21974073)
- Education Department of Hunan Province (20A299)
This publication has 50 references indexed in Scilit:
- Liposome–Quantum Dot Complexes Enable Multiplexed Detection of Attomolar DNAs without Target AmplificationJournal of the American Chemical Society, 2013
- Highly Fluorescent Semiconducting Polymer Dots for Biology and MedicineAngewandte Chemie-International Edition, 2013
- Single Gold Nanoparticles as Real‐Time Optical Probes for the Detection of NADH‐Dependent Intracellular Metabolic Enzymatic PathwaysAngewandte Chemie-International Edition, 2011
- Ultrabright and Bioorthogonal Labeling of Cellular Targets Using Semiconducting Polymer Dots and Click ChemistryAngewandte Chemie-International Edition, 2010
- Glucosamine hydrochloride functionalized tetraphenylethylene: A novel fluorescent probe for alkaline phosphatase based on the aggregation-induced emissionChemical Communications, 2010
- Single Quantum Dot-Based Nanosensor for Multiple DNA DetectionAnalytical Chemistry, 2010
- Combining Alkaline Phosphatase Treatment and Hybrid Linear Ion Trap/Orbitrap High Mass Accuracy Liquid Chromatography−Mass Spectrometry Data for the Efficient and Confident Identification of Protein PhosphorylationAnalytical Chemistry, 2009
- Mussel-Inspired Surface Chemistry for Multifunctional CoatingsScience, 2007
- Alkaline Phosphatase-Catalyzed Silver Deposition for Electrochemical DetectionAnalytical Chemistry, 2007
- Horseradish peroxidase sol–gel immobilized for chemiluminescence measurements of alkaline-phosphatase activitySensors and Actuators B: Chemical, 2001