Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
Open Access
- 12 February 2021
- journal article
- research article
- Published by MDPI AG in Microorganisms
- Vol. 9 (2), 363
- https://doi.org/10.3390/microorganisms9020363
Abstract
Leishmania amazonensis parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB/c mice are highly susceptible to L. amazonensis challenge due to their inability to mount parasite-dependent IFN-γ-mediated responses. Here, we analyzed the capacity of a single administration of the LiΔHSP70-II genetically-modified attenuated L. infantum line in preventing cutaneous leishmaniasis in mice challenged with L. amazonensis virulent parasites. In previous studies, this live attenuated vaccine has demonstrated to induce long-protection against murine leishmaniasis due to Old World Leishmania species. Vaccinated mice showed a reduction in the disease evolution due to L. amazonensis challenge, namely reduction in cutaneous lesions and parasite burdens. In contrast to control animals, after the challenge, protected mice showed anti-Leishmania IgG2a circulating antibodies accompanied to the induction of Leishmania-driven specific IFN-γ systemic response. An analysis performed in the lymph node draining the site of infection revealed an increase of the parasite-specific IFN-ϒ production by CD4+ and CD8+ T cells and a decrease in the secretion of IL-10 against leishmanial antigens. Since the immunity caused by the inoculation of this live vaccine generates protection against different forms of murine leishmaniasis, we postulate LiΔHSP70-II as a candidate for the development of human vaccines.Keywords
Funding Information
- Ministerio de Ciencia e Innovación (FISPI11/00095 and FISPI14/00366 FEDER FUNDING, RYC-2016-19463 and RTI2018-343, FPI grant to E.H.G.)
- Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/008-FEDER)
- Fundación Ramón Areces (CBMSO Institutional Grant)
- Banco Santander (CBMSO Institutiional Grant)
This publication has 54 references indexed in Scilit:
- Effect of essential oils on Leishmania amazonensis: a systematic reviewParasitology, 2020
- What makes mucosal and anergic diffuse cutaneous leishmaniases so clinically and immunopathogically different? A review in BrazilTransactions of the Royal Society of Tropical Medicine and Hygiene, 2019
- Total Leishmania antigens with Poly(I:C) induce Th1 protective responseParasite Immunology, 2017
- Cutaneous leishmaniasis: immune responses in protection and pathogenesisNature Reviews Immunology, 2016
- Immunization with antigenic extracts of Leishmania associated with Montanide ISA 763 adjuvant induces partial protection in BALB/c mice against Leishmania (Leishmania) amazonensis infectionJournal of Microbiology, Immunology and Infection, 2014
- T Helper1/T Helper2 Cells and Resistance/Susceptibility to Leishmania Infection: Is This Paradigm Still Relevant?Frontiers in Immunology, 2012
- Immunological characteristics of experimental murine infection with Leishmania (Leishmania) amazonensisVeterinary Parasitology, 2008
- Vaccine-induced protection against Leishmania amazonensis is obtained in the absence of IL-12/23p40Immunology Letters, 2006
- Clinical and immunopathological spectrum of American cutaneous leishmaniasis with special reference to the disease in Amazonian Brazil: a reviewMemórias do Instituto Oswaldo Cruz, 2004
- T cell responses to crude and defined leishmanial antigens in patients from the Lower Amazon region of Brazil infected with different species of Leishmania of the subgenera Leishmania and VianniaParasite Immunology, 1998