Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020
Open Access
- 11 February 2021
- journal article
- research article
- Published by European Centre for Disease Control and Prevention (ECDC) in Eurosurveillance
- Vol. 26 (6), 2002079
- https://doi.org/10.2807/1560-7917.es.2021.26.6.2002079
Abstract
We report the performance of a variety of commercially available SARS-CoV-2 PCR kits, used in several different sites across Ireland to determine if Ct values across platforms are comparable. We also investigate whether a Ct value, a surrogate for calculated viral loads in the absence of viral culture of > 34 can be used to exclude SARS-CoV-2 infection and its complications. We found a variation in Ct values from different assays for the same calculated viral load; this should be taken into consideration for result interpretation.Keywords
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