Efficient biorefinery of whole cassava for citrate production using Aspergillus niger mutated by atmospheric and room temperature plasma and enhanced co‐saccharification strategy
- 20 January 2021
- journal article
- research article
- Published by Wiley in Journal of the Science of Food and Agriculture
- Vol. 101 (11), 4613-4620
- https://doi.org/10.1002/jsfa.11104
Abstract
BACKGROUND The non‐grain crop cassava has attracted intense attention in the biorefinery process. However, efficient biorefinery of whole cassava is faced with some challenges due to the existence of strain inhibition and refractory cellulose during the citrate production process. RESULTS Here, a novel breeding method – atmospheric and room temperature plasma (ARTP) – was applied for strain improvement of citrate‐producing strain Aspergillus niger from whole cassava. The citrate yield of the mutant obtained using ARTP mutagenesis increased by 36.5% in comparison with the original strain. Moreover, citric acid fermentation was further improved on the basis of an enhanced co‐saccharification strategy by supplementing glucoamylase and cellulase. The fermentation efficiency increased by 35.8% with a 17.0 g L−1 reduction in residual sugar on a pilot scale. CONCLUSIONS All these results confirmed that a combination of the novel breeding method and enhanced co‐saccharification strategy could be used to efficiently refine whole cassava. The results also provide inspiration for the production of value‐added products and waste disposal in agro‐based industries. © 2021 Society of Chemical IndustryKeywords
Funding Information
- Jiangnan University (KLCCB‐KF202005)
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