Integrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava
- 5 August 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Plant Molecular Biology
- Vol. 109 (3), 249-269
- https://doi.org/10.1007/s11103-020-01033-8
Abstract
Key Message Integrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava. Abstract Tuberous root formation is a complex process consisting of phase changes as well as cell division and elongation for radial growth. We performed an integrated analysis to clarify the relationships among metabolites, phytohormones, and gene transcription during tuberous root formation in cassava (Manihot esculenta Crantz). We also confirmed the effects of the auxin (AUX), cytokinin (CK), abscisic acid (ABA), jasmonic acid (JA), gibberellin (GA), brassinosteroid (BR), salicylic acid, and indole-3-acetic acid conjugated with aspartic acid on tuberous root development. An integrated analysis of metabolites and gene expression indicated the expression levels of several genes encoding enzymes involved in starch biosynthesis and sucrose metabolism are up-regulated during tuberous root development, which is consistent with the accumulation of starch, sugar phosphates, and nucleotides. An integrated analysis of phytohormones and gene transcripts revealed a relationship among AUX signaling, CK signaling, and BR signaling, with AUX, CK, and BR inducing tuberous root development. In contrast, ABA and JA inhibited tuberous root development. These phenomena might represent the differences between stem tubers (e.g., potato) and root tubers (e.g., cassava). On the basis of these results, a phytohormonal regulatory model for tuberous root development was constructed. This model may be useful for future phytohormonal studies involving cassava.Keywords
Funding Information
- CONCERT-Japan (JPMJSC16C4)
This publication has 72 references indexed in Scilit:
- Transcriptome Analysis Using a High-Density Oligomicroarray under Drought Stress in Various Genotypes of Cassava: An Important Tropical CropDNA Research, 2012
- Gibberellin A1 Metabolism Contributes to the Control of Photoperiod-Mediated Tuberization in PotatoPLOS ONE, 2011
- Functional Diversity of Isoamylase Oligomers: The ISA1 Homo-Oligomer Is Essential for Amylopectin Biosynthesis in Rice EndospermPlant Physiology, 2011
- Covering Chemical Diversity of Genetically-Modified Tomatoes Using Metabolomics for Objective Substantial Equivalence AssessmentPLOS ONE, 2011
- Expression Profiling of Cassava Storage Roots Reveals an Active Process of Glycolysis/GluconeogenesisFJournal of Integrative Plant Biology, 2011
- AHD2.0: an update version of Arabidopsis Hormone Database for plant systematic studiesNucleic Acids Research, 2010
- Highly Sensitive and High-Throughput Analysis of Plant Hormones Using MS-Probe Modification and Liquid ChromatographyTandem Mass Spectrometry: An Application for Hormone Profiling in Oryza sativaPlant and Cell Physiology, 2009
- IbMADS1 (Ipomoea batatas MADS-box 1 gene) is Involved in Tuberous Root Initiation in Sweet Potato (Ipomoea batatas)Annals of Botany, 2008
- In vitro Cultured Primary Roots Derived from Stem Segments of Cassava ( Manihot esculenta ) Can Behave Like Storage OrgansAnnals of Botany, 2007
- Diurnal Changes in the Transcriptome Encoding Enzymes of Starch Metabolism Provide Evidence for Both Transcriptional and Posttranscriptional Regulation of Starch Metabolism in Arabidopsis LeavesPlant Physiology, 2004