Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device
Top Cited Papers
- 3 December 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biomedical Engineering
- Vol. 4 (12), 1159-1167
- https://doi.org/10.1038/s41551-020-00654-0
Abstract
The diagnosis of severe acute respiratory syndrome 2 (SARS-CoV-2) infection by quantitative PCR with reverse transcription (RT–qPCR) typically involves bulky instrumentation in centralized laboratories and an assay time of 1–2 h. Here, we show that SARS-CoV-2 RNA can be detected in 17 min via a portable device integrating reverse transcription, fast thermocycling (via plasmonic heating through magneto-plasmonic nanoparticles) and in situ fluorescence detection following magnetic clearance of the nanoparticles. The device correctly classified all nasopharyngeal, oropharyngeal and sputum samples from 75 patients with COVID-19 and 75 healthy controls, with good concordance in fluorescence intensity with standard RT–qPCR (Pearson coefficients > 0.7 for the N1, N2 and RPP30 genes). Fast, portable and automated nucleic acid detection should facilitate testing at the point of care.Keywords
Funding Information
- Institute for Basic Science (IBS-R026-D1)
This publication has 50 references indexed in Scilit:
- Plasmonics simulations with the MNPBEM toolbox: Consideration of substrates and layer structuresComputer Physics Communications, 2015
- Ultrafast photonic PCRLight: Science & Applications, 2015
- Fast and Transparent Adaptive Lens Based on Plasmonic HeatingACS Photonics, 2015
- Plasmon-induced hot carrier science and technologyNature Nanotechnology, 2015
- Nanoparticles Heat through Light LocalizationNano Letters, 2014
- Photoinduced Heating of Nanoparticle ArraysACS Nano, 2013
- Plasmonics for future biosensorsNature Photonics, 2012
- The design and application of fluorophore–gold nanoparticle activatable probesPhysical Chemistry Chemical Physics, 2011
- Plasmonic coupling in noble metal nanostructuresChemical Physics Letters, 2010
- Retarded field calculation of electron energy loss in inhomogeneous dielectricsPhysical Review B, 2002