vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure
Open Access
- 9 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-18
- https://doi.org/10.1038/s41598-021-91289-x
Abstract
One of the serious public health concerns is food contaminated with pathogens and their vital activity products such as toxins. Bacillus cereus group of bacteria includes well-known pathogenic species such as B. anthracis, B. cereus sensu stricto (ss), B. cytotoxicus and B. thuringiensis. In this report, we describe the Bacillus phages vB_BcM_Sam46 and vB_BcM_Sam112 infecting species of this group. Electron microscopic analyses indicated that phages Sam46 and Sam112 have the myovirus morphotype. The genomes of Sam46 and Sam112 comprise double-stranded DNA of 45,419 bp and 45,037 bp in length, respectively, and have the same GC-content. The genome identity of Sam46 and Sam112 is 96.0%, indicating that they belong to the same phage species. According to the phylogenetic analysis, these phages form a distinct clade and may be members of a new phage genus, for which we propose the name ‘Samaravirus’. In addition, an interesting feature of the Sam46 and Sam112 phages is the unusual structure of their small terminase subunit containing N-terminal FtsK_gamma domain.Funding Information
- Russian Foundation for Basic Research (19-04-00300)
- Ministry of Science and Higher Education of the Russian Federation (075-03-2019-525/2 (AAAA-A19-119120390010-1))
This publication has 74 references indexed in Scilit:
- SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell SequencingJournal of Computational Biology, 2012
- Fast gapped-read alignment with Bowtie 2Nature Methods, 2012
- Easyfig: a genome comparison visualizerBioinformatics, 2011
- Aligning Short Sequencing Reads with BowtieCurrent Protocols in Bioinformatics, 2010
- A low-polynomial algorithm for assembling clusters of orthologous groups from intergenomic symmetric best matchesBioinformatics, 2010
- Determining DNA Packaging Strategy by Analysis of the Termini of the Chromosomes in Tailed-Bacteriophage VirionsPublished by Springer Science and Business Media LLC ,2009
- Molecular Mechanism of Sequence-Directed DNA Loading and Translocation by FtsKMolecular Cell, 2008
- Sequence-directed DNA export guides chromosome translocation during sporulation in Bacillus subtilisNature Structural & Molecular Biology, 2008
- The FtsK γ domain directs oriented DNA translocation by interacting with KOPSNature Structural & Molecular Biology, 2006
- Application of Phylogenetic Networks in Evolutionary StudiesMolecular Biology and Evolution, 2005