A facile, versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity, cell viability and proliferation
Open Access
- 25 April 2021
- journal article
- research article
- Published by Oxford University Press (OUP) in Regenerative Biomaterials
- Vol. 8 (3), rbab026
- https://doi.org/10.1093/rb/rbab026
Abstract
Both of the long-term fidelity and cell viability of three-dimensional (3D)-bioprinted constructs are essential to precise soft tissue repair. However, the shrinking/swelling behavior of hydrogels brings about inadequate long-term fidelity of constructs, and bioinks containing excessive polymer are detrimental to cell viability. Here, we obtained a facile hydrogel by introducing 1% aldehyde hyaluronic acid (AHA) and 0.375% N-carboxymethyl chitosan (CMC), two polysaccharides with strong water absorption and water retention capacity, into classic gelatin (GEL, 5%)–alginate (ALG, 1%) ink. This GEL–ALG/CMC/AHA bioink possesses weak temperature dependence due to the Schiff base linkage of CMC/AHA and electrostatic interaction of CMC/ALG. We fabricated integrated constructs through traditional printing at room temperature and in vivo simulation printing at 37°C. The printed cell-laden constructs can maintain subaqueous fidelity for 30 days after being reinforced by 3% calcium chloride for only 20 s. Flow cytometry results showed that the cell viability was 91.38 ± 1.55% on day 29, and the cells in the proliferation plateau at this time still maintained their dynamic renewal with a DNA replication rate of 6.06 ± 1.24%. This work provides a convenient and practical bioink option for 3D bioprinting in precise soft tissue repair.Keywords
Funding Information
- National Natural Science Foundation of China (81771239, 52075285)
- Science and Technology Program of Guangzhou, China (201604040002)
- Key-Area Research and Development Program of Guangdong Province, China (2020B090923003)
- Key Research and Development Projects of People’s Liberation Army, China (BWS17J036)
This publication has 83 references indexed in Scilit:
- 3D Bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogelsJournal of Biomedical Materials Research Part A, 2012
- Highly stretchable and tough hydrogelsNature, 2012
- Injectable in situ forming biodegradable chitosan–hyaluronic acid based hydrogels for cartilage tissue engineeringBiomaterials, 2009
- A chemical method for fast and sensitive detection of DNA synthesis in vivoProceedings of the National Academy of Sciences of the United States of America, 2008
- Maintaining dimensions and mechanical properties of ionically crosslinked alginate hydrogel scaffolds in vitroJournal of Biomedical Materials Research Part A, 2007
- Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cellsProceedings of the National Academy of Sciences of the United States of America, 2007
- Physically crosslinked alginate/N,O-carboxymethyl chitosan hydrogels with calcium for oral delivery of protein drugsBiomaterials, 2005
- Synthesis of cross-linked poly(aldehyde guluronate) hydrogelsPolymer, 1999
- The Cell Adhesion Molecule, GP116, Is a New CD44 Variant (ex14/v10) Involved in Hyaluronic Acid Binding and Endothelial Cell ProliferationOnline Journal of Public Health Informatics, 1996
- Role of CD44 in the reaction of vascular smooth muscle cells to arterial wall injury.JCI Insight, 1996