Simultaneous Imaging of Endogenous Survivin mRNA and On-Demand Drug Release in Live Cells by Using a Mesoporous Silica Nanoquencher
- 24 May 2017
- Vol. 13 (27)
- https://doi.org/10.1002/smll.201700569
Abstract
The design of multifunctional drug delivery systems capable of simultaneous target detection, imaging, and therapeutics in live mammalian cells is critical for biomedical research. In this study, by using mesoporous silica nanoparticles (MSNs) chemically modified with a small-molecule dark quencher, followed by sequential drug encapsulation, MSN capping with a dye-labeled antisense oligonucleotide, and bioorthogonal surface modification with cell-penetrating poly(disulfide)s, the authors have successfully developed the first mesoporous silica nanoquencher (qMSN), characterized by high drug-loading and endocytosis-independent cell uptake, which is able to quantitatively image endogenous survivin mRNA and release the loaded drug in a manner that depends on the survivin expression level in tumor cells. The authors further show that this novel drug delivery system may be used to minimize potential cytotoxicity encountered by many existing small-molecule drugs in cancer therapy.Funding Information
- National Medical Research Council (CBRG/0038/2013)
- Ministry of Education of the Singapore government (MOE2013-T2-1-048)
- National Natural Science Foundation of China (51403065, sklssm201730)
This publication has 56 references indexed in Scilit:
- Gated Materials for On-Command Release of Guest MoleculesChemical Reviews, 2016
- Emerging Biosensing Approaches for microRNA AnalysisAnalytical Chemistry, 2015
- Engineered Hybrid Nanoparticles for On-Demand Diagnostics and TherapeuticsAccounts of Chemical Research, 2015
- Target identification of natural products and bioactive compounds using affinity-based probesNatural Product Reports, 2015
- Molecular and supramolecular switches on mesoporous silica nanoparticlesChemical Society Reviews, 2015
- MicroRNA: Function, Detection, and BioanalysisChemical Reviews, 2013
- Gated Silica Mesoporous Supports for Controlled Release and Signaling ApplicationsAccounts of Chemical Research, 2012
- Mesoporous silica nanoparticles in biomedical applicationsChemical Society Reviews, 2012
- Identification of the cellular targets of bioactive small organic molecules using affinity reagentsChemical Society Reviews, 2008
- Target deconvolution strategies in drug discoveryNature Reviews Drug Discovery, 2007