Choice of cystometric technique impacts detrusor contractile dynamics in wistar rats
Open Access
- 1 January 2021
- journal article
- research article
- Published by Wiley in Physiological Reports
- Vol. 9 (2), e14724
- https://doi.org/10.14814/phy2.14724
Abstract
The objective of the current animal study was to investigate factors contributing to the different phases of the cystometrogram (CMG) in order to address disparities in research data reported in the current literature. Three experiments in 20 female Wistar rats were designed to investigate (1) the effects of anesthesia on the contractile pattern of the bladder during micturition; (2) the impact of the physical characteristics of the CMG technique upon the accuracy of intra-vesical pressure recordings; and (3) identification of physiological and methodological factors associated with the emptying and rebound phases during CMG. Variables tested included awake versus urethane-anesthetized conditions, use of a single catheter for both filling and intra-vesical pressure (Pves) recording versus a separate two catheter approach, and comparisons between ureter, bladder dome, and urethral catheter placements. Both awake and anesthetized conditions contributed to variations in the shape and magnitude of the CMG pressure curves. In addition, catheter size, acute incision of the bladder dome for catheter placement, use of the same catheter for filling and Pves recordings, as well as the placement and positioning of the tubing, all contributed to alterations of the physiological properties and characteristic of the various CMG phases, including the frequent occurrence of an artificial rebound during the third phase of micturition. The present results demonstrate how different experimental conditions lead not only to variability in Pves curves, but consistency of the measurements as well, which needs to be accounted for when interpreting CMG outcome data.Funding Information
- Kentucky Spinal Cord and Head Injury Research Trust (17‐5)
- Consejo Nacional de Ciencia y Tecnología
This publication has 64 references indexed in Scilit:
- Neural control of the female urethral and anal rhabdosphincters and pelvic floor musclesAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2010
- Sprouting of substance P-expressing primary afferent central terminals and spinal micturition reflex NK1 receptor dependence after spinal cord injuryAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2008
- Differential effects of urethane and isoflurane on external urethral sphincter electromyography and cystometry in ratsAmerican Journal of Physiology-Renal Physiology, 2008
- Activation of Urothelial Transient Receptor Potential Vanilloid 4 by 4α-Phorbol 12,13-Didecanoate Contributes to Altered Bladder Reflexes in the RatThe Journal of pharmacology and experimental therapeutics, 2007
- Is abdominal wall contraction important for normal voiding in the female rat?BMC Urology, 2007
- Serotonergic drugs and spinal cord transections indicate that different spinal circuits are involved in external urethral sphincter activity in ratsAmerican Journal of Physiology-Renal Physiology, 2007
- Effects of anesthesia on cystometry and leak point pressure of the female ratLife Sciences, 2001
- Simultaneous recording of vesical and urethral pressure in urethane-anesthetized rats: Effect of neuromuscular blocking agents on the activity of the external urethral sphincterJournal of Pharmacological Methods, 1991
- New method for recording cystometrograms in conscious, freely moving ratsJournal of Pharmacological Methods, 1988
- The nonstop transvesical cystometrogram in urethane-anesthetized rats: A simple procedure for quantitative studies on the various phases of urinary bladder voiding cycleJournal of Pharmacological Methods, 1986