Single Biomolecule Imaging by Electrochemiluminescence
- 22 October 2021
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 143 (43), 17910-17914
- https://doi.org/10.1021/jacs.1c06673
Abstract
Herein, a single biomolecule is imaged by electrochemiluminescence (ECL) using Ru(bpy)32+-doped silica/Au nanoparticles (RuDSNs/AuNPs) as the ECL nanoemitters. The ECL emission is confined to the local surface of RuDSNs leading to a significant enhancement in the intensity. To prove the concept, a single protein molecule at the electrode is initially visualized using the as-prepared RuDSN/AuNPs nanoemitters. Furthermore, the nanoemitter-labeled antibody is linked at the cellular membrane to image a single membrane protein at one cell, without the interference of current and optical background. The success in single-biomolecule ECL imaging solves the long-lasting task in the ultrasensitive ECL analysis, which should be able to provide more elegant information about the protein in cellular biology.Keywords
Funding Information
- Agence Nationale de la Recherche (ELISE - ANR-21-CE42)
- Ministry of Science and Technology of the People's Republic of China (2017YFC0906700)
- National Natural Science Foundation of China (21775028, 21934001, 22025403)
This publication has 41 references indexed in Scilit:
- Carbon Electrode–Molecule Junctions: A Reliable Platform for Molecular ElectronicsAccounts of Chemical Research, 2015
- Observation of Single-Protein and DNA Macromolecule Collisions on UltramicroelectrodesJournal of the American Chemical Society, 2015
- Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassaysChemical Science, 2014
- Breaking the concentration limit of optical single-molecule detectionChemical Society Reviews, 2014
- Dynamic Evaluation of Cell Surface N-Glycan Expression via an Electrogenerated Chemiluminescence Biosensor Based on Concanavalin A-Integrating Gold-Nanoparticle-Modified Ru(bpy)32+-Doped Silica NanoprobeAnalytical Chemistry, 2013
- Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopyProceedings of the National Academy of Sciences of the United States of America, 2012
- Development of a New Device for Ultrasensitive Electrochemiluminescence Microscopy ImagingAnalytical Chemistry, 2009
- Multiplexed Sandwich Immunoassays Using Electrochemiluminescence Imaging Resolved at the Single Bead LevelJournal of the American Chemical Society, 2009
- Counting Low-Copy Number Proteins in a Single CellScience, 2007
- Aggregation-Induced Folding of a De Novo Designed Polypeptide Immobilized on Gold NanoparticlesJournal of the American Chemical Society, 2006