Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells
Open Access
- 27 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cell Death & Disease
- Vol. 11 (4), 1-11
- https://doi.org/10.1038/s41419-020-2459-3
Abstract
Cold atmospheric plasma (CAP) has been proposed as a novel promising anti-cancer treatment modality. Apoptosis and necrosis have been revealed in CAP-induced cell death, but whether CAP induces pyroptosis, another kind of programmed cell death is still unknown. In the present study, we first reported that CAP effectively induced pyroptosis in a dose-dependent manner in Gasdermin E (GSDME) high-expressed tumor cell lines. Interestingly, the basal level of GSDME protein was positively correlated with the sensitivity to CAP in three selected cancer cell lines, implying GSDME might be a potential biomarker of prognosis in the forthcoming cancer CAP treatment. Moreover, our study revealed that CAP-induced pyroptosis depended on the activation of mitochondrial pathways (JNK/cytochrome c/caspase-9/caspase-3) and the cleavage of GSDME but not Gasdermin D (GSDMD). ROS generation induced by CAP was identified to initiate the pyroptotic signaling. These results complemented our knowledge on CAP-induced cell death and provide a strategy to optimize the effect of CAP cancer treatment.Keywords
This publication has 46 references indexed in Scilit:
- Molecular mechanisms of non-thermal plasma-induced effects in cancer cellsBiological Chemistry, 2018
- Chemically different non-thermal plasmas target distinct cell death pathwaysScientific Reports, 2017
- Cold atmospheric plasma, a novel promising anti-cancer treatment modalityOncotarget, 2016
- Non-thermal gas plasma-induced endoplasmic reticulum stress mediates apoptosis in human colon cancer cellsOncology Reports, 2016
- Reactive species in non-equilibrium atmospheric-pressure plasmas: Generation, transport, and biological effectsPhysics Reports, 2016
- Toward understanding the selective anticancer capacity of cold atmospheric plasma—A model based on aquaporins (Review)Biointerphases, 2015
- Plasma-activated medium induces A549 cell injury via a spiral apoptotic cascade involving the mitochondrial–nuclear networkFree Radical Biology & Medicine, 2015
- A Review on the Selective Apoptotic Effect of Nonthermal Atmospheric-Pressure Plasma on Cancer CellsPlasma Medicine, 2014
- ROS implication in a new antitumor strategy based on non‐thermal plasmaInternational Journal of Cancer, 2011
- Induction of cell growth arrest by atmospheric non-thermal plasma in colorectal cancer cellsJournal of Biotechnology, 2010