The proximity of the G-quadruplex to hemin impacts the intrinsic DNAzyme activity in mitochondria
- 9 February 2021
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 57 (24), 3038-3041
- https://doi.org/10.1039/d0cc08316j
Abstract
The DNAzyme activity of G-quadruplex/hemin in mitochondria has not been characterized. Herein, we report an unexpected difference in the DNAzyme activity between IN VITRO assays and in mitochondria. Molecular dynamic simulations illustrate how the interaction of the G-quadruplex with hemin may modulate the DNAzyme activity. These results might facilitate a better understanding of the catalytic mechanism of the DNAzyme and help the rational design of stable and active DNAzymes suitable for intracellular catalysis.Keywords
Funding Information
- National Natural Science Foundation of China (11875269, 21574136)
- Chinese Academy of Sciences
- Youth Innovation Promotion Association (2015008)
This publication has 34 references indexed in Scilit:
- G-Quadruplex-Based Nanoscale Coordination Polymers to Modulate Tumor Hypoxia and Achieve Nuclear-Targeted Drug Delivery for Enhanced Photodynamic TherapyNano Letters, 2018
- G‐Quadruplex Secondary Structure Obtained from Circular Dichroism SpectroscopyAngewandte Chemie, 2018
- A Thermophilic Tetramolecular G‐Quadruplex/Hemin DNAzymeAngewandte Chemie, 2017
- RNA G-quadruplexes are globally unfolded in eukaryotic cells and depleted in bacteriaScience, 2016
- Metal ions modulate the conformation and stability of a G-quadruplex with or without a small-molecule ligandMetallomics, 2015
- DNA Switches: From Principles to ApplicationsAngewandte Chemie, 2014
- C9orf72 nucleotide repeat structures initiate molecular cascades of diseaseNature, 2014
- Quantitative visualization of DNA G-quadruplex structures in human cellsNature Chemistry, 2013
- Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solutionNucleic Acids Research, 2006
- Crystal structure of parallel quadruplexes from human telomeric DNANature, 2002