MiR‐342 attenuates lipopolysaccharide‐induced acute lung injury via inhibiting MAPK1 expression

Abstract

MiRNAs and mitogen‐activated protein kinase (MAPK) are reported as the crucial regulators of inflammatory responses in acute lung injury (ALI). This study will explore the role of miR‐342/MAPK1 axis in regulation of lipopolysaccharide (LPS)‐induced ALI. We found miR‐342 was downregulated in LPS‐induced A549 cells compared with control group with DMSO, accompanied by elevated inflammatory cytokines and apoptosis. Overexpression of miR‐342 reduced LPS‐induced inflammatory responses and apoptosis in LPS‐stimulated A549 cells, and had a protective role in LPS‐treated mice with ALI by decreasing levels of inflammatory cytokines, improving survival of mice with ALI, and ameliorating the lung permeability. Dual‐luciferase reporter gene assay demonstrated that miR‐342 regulated the expression of MAPK1 by directly targeting its 3’ untranslated region (3’‐UTR). Mechanistically, MAPK1 silencing abrogated LPS‐induced inflammatory injury in A549 cells, and partially enhanced the protective effect of miR‐342. Therefore, miR‐342 attenuates LPS‐induced ALI by targeting MAPK1 expression, thereby protecting against A549 cell injury induced by LPS and lung injury of mice with ALI.